聚四氟乙烯膜管内植入自体许旺氏细胞桥接面神经的实验研究

目的　观察聚四氟乙烯膜管内植入自体许旺氏细胞桥接修复面神经缺损的效果。方法　采用聚四氟乙 烯膜管桥接家兔面神经1.0 cm的缺损,将快速提取获得的自体许旺氏细胞植入膜管内作为实验组,选择单纯聚四 氟乙烯膜管桥接面神经缺损为对照组,用电生理测试和组织学观察等方法对修复后不同时期神经再生情况进行评 价。结果　实验组各时期神经再生情况均优于对照组,其中实验组术后16周时面神经传导速度为29·70 m/s,而对 照组为23·00 m/s,统计学处理两者有显著性差异(P<0·05)。结论　一定数量有活力的自体许旺氏细胞植入聚四 氟乙烯膜管制成的面神经再生室内,可以获得较好的面神经修复效果。

Experimental Study of Facial Nerve Regeneration Through Polytetraflouroethylene Tube with Schwann Cells Self-planted

Objective To observe the effect of using polytetraflouroethylene(e-PTFE) tube with self-Sc hwann cells implanted to repair facial nerve defect.Methods Enzymatic digest method was used to get pure Schwann cells in short time. The e-PTFE membranetube was used to bridge the 1.0cm defect of facial nerve and pure self-Schwann cells were injected into the tube. As control group, the e-PTFE tube without self-Schwann cells was used in the same way. Electric physiological and histological examinations were taken in different times.Results The effect of nerve regeneration of the experimental group was better than control group at any time. The nerve conduction velocity of the experimental group was 29.70 m/s in the 16 {th} week, while the control groups was 23.00 m/s respectively at the same time.Conclusion It is possible to obtain sufficient active Schwann cells by enzymatic digest met hod. Using e-PTFE tube to bridge the defect of facial nerve with self-Schwann cells implanted can get effect of nerve regeneration.