流体切应力作用下乙醇对血管内皮细胞HSP70表达的影响

目的探讨在流体切应力作用下不同浓度的乙醇对血管内皮细胞HSP70表达的影响。方法不同浓度的乙醇分别在静止状态下和1 Pa切应力下作用于培养的EA．Hy926内皮细胞1h、2h、4h、6h，不加乙醇组为对照组，用免疫组化SP法观察内皮细胞HSP70的表达。结果对照组在静止状态下无HSP70的表达，在1 Pa切应力作用4h后可见表达；50mg／dL乙醇组不管在有无切应力作用下表达HSP70与对照组的差异均无统计学意义；150mg／dL和300mg／dL乙醇组在静止状态下作用于内皮细胞4h和2h可见HSP70表达，在1 Pa切应力作用下表达明显增强。绪论中、高浓度的乙醇与流体切应力的联合作用能明显增强内皮细胞HSP70的表达，同时与作用时间也有密切关系。

A Study on the Expression of HSP70 in Endothelial Cells Pretreated with Ethanol and Fluid Shear Stress

Objective To detect whether ethanol can affect the expression of HSP70 in endothelial cells under fluid shear stress.Methods Ethanol at different concentrations was added to the culture medium of endothelial cells,EA.Hy926,which was treated statically or under 1Pa fluid shear stress.After the incubation of 1 h,2 h,4 h and 6 h,the expression of HSP70 was detected by immunohistochemical method(SP).Results In the control group,the expression of HSP70 was negative under static state,while it was positive under 1Pa fluid shear stress lasting 4 h even without ethanol.No statistic difference was found between the 50 mg/dL ethanol group and the control group with the same treatment time of fluid shear stress.HSP70 expression was found under static state with 150 mg/dL ethanol after 4 h or 300 mg/dL ethanol after 2 h respectively.The expression increased greatly under 1Pa fluid shear stress in the same ethanol concentrations.Conclusion Medium to high ethanol concentration in coordination with fluid shear stress can strongly stimulates the expression of HSP70 by a kinetic mechanism of time-dependent.