常用维生素对人前脂肪细胞增殖和分化的作用

目的研究维生素(A、B、C、D、E、K各族)对前脂肪细胞增殖和分化的作用。方法培养人前脂肪细胞，在其生长的不同阶段分别加入各种维生素，观察其对细胞的增殖、分化过程中的磷酸甘油脱氢酶(GPDH)和细胞内脂肪积聚的作用。结果维生素A对人前脂肪细胞增殖和分化均有抑制作用，维生素B6对人前脂肪细胞的分化有促进作用，维生素C对人前脂肪细胞增殖和分化均有明显的促进作用，维生素D3对人前脂肪细胞的分化有抑制作用，维生素E对人前脂肪细胞的分化有较强的抑制作用，维生素K1对人前脂肪细胞分化有极强的刺激作用，维生素K3对人前脂肪细胞的增殖和分化均有抑制作用。未发现其他维生素对人前脂肪细胞的明显作用。结论该研究结果可为人体合理地摄入维生素提供理论指导。

Effects of vitamines on human preadipocyte proliferation and differentiation

ObjectiveTo study the effects of vitamins on human pr eadipocyte proliferation and differentiation. MethodsVit amins (A, B, C, D, E and K families) were added to the culture media of human p readipocytes, then the proliferation of cells, the expression of GPDH and lipid droplet accumulation in the cytoplasm were recorded. ResultsVitamin A inhibited human preadipocyte proliferation and differentiation. Vi tamin C enhanced differentiation and proliferation. Vitamin B 6 stimulated diff erentiation. vitamin D 3 inhibited differentiation. Vitamin E had a strong inhi bitory effect on human preadipocyte differentiation. Vitamin K 1 had an unexpec tedly great stimulatory effect on human preadipocyte differentiation. Vitamin K 3 inhibited both human preadipocyte proliferation and differentiation. Other v itamins tested showed no noticeable effects. ConclusionT hese data are advisory to our balanced daily intake of vitamins.