外周血CK20 mRNA表达与结直肠癌临床病理和预后的关系

目的:探讨外周静脉血中CK20 mRNA表达及其与结直肠癌临床病理及预后的关系.方法:选取结直肠癌术前患者61例、健康志愿者20例和结直肠息肉患者10例,采用RT-PCR法检测其外周静脉血中CK20 mRNA的表达,并结合其临床病理特点和随访资料进行综合分析.结果:61例结直肠癌患者术前外周血CK20 mRNA阳性率为41.0%(25/61);其表达与肿瘤TNM分期、浸润深度、远处转移和区域淋巴结转移有关(P<0.05).20例健康志愿者和10例结直肠息肉患者外周血均无CK20 mRNA表达.在61例结直肠癌患者中,术后46mo完整随访的有37例,其中有17例死亡,有10例外周血CK20 mRNA表达阳性.结论:外周静脉血中CK20 mRNA的表达与结直肠癌患者的肿瘤分期和浸润转移有关.血液中CK20是反映结直肠癌患者发生肿瘤微转移较为特异的肿瘤标志物,外周血液中CK20 mRNA的表达可作为评估患者预后的指标.

Correlation between expression of CK20 mRNA in peripheral blood and clinicopathological features and prognosis in patients with colorectal cancer

AIM:To investigate the clinical significance of cytokeratin 20(CK20) mRNA expression in peripheral venous blood of patients with colorectal carcinoma.METHODS:The expression of CK20 mRNA was detected by RT-PCR in peripheral venous blood samples from 20 healthy volunteers,10 patients with colorectal polyps,and 61 patients with colorectal cancer.RESULTS:The positive rate of CK20 mRNA in peripheral venous blood of patients with colorectal cancer was 41.0%(25/61).CK20 mRNA expression in peripheral venous blood was asso-ciated with TNM stage,local lymph node metastasis,distant metastasis,and depth of invasion(all P < 0.05).The expression of CK20 mRNA was not detected in peripheral venous blood of healthy volunteers and patients with colorectal polyps.Seventeen cases died during the follow-up period,of whom 10 had positive expression of CK20 in peripheral blood.CONCLUSION:CK20 is a specific marker for blood micrometastasis of colorectal cancer.The expression of CK20 mRNA in the blood of patients with colorectal cancer is related with the invasion and metastasis of colorectal cancer.Detection of CK20 mRNA in peripheral blood seems to be of relevance for prognosis in colorectal cancer.