南海海洋真菌Penicillium sp. FS60的次级代谢产物研究

目的:研究南海海洋真菌Penicillium sp.FS60的次级代谢产物及其细胞毒活性。方法:采用正相硅胶柱、反相硅胶柱、凝胶柱、HPLC和薄层制备等色谱技术和重结晶对菌株FS60的发酵产物进行分离纯化,并通过波谱分析进行结构鉴定;以神经胶质瘤细胞SF-268、乳腺癌细胞MCF-7、大细胞肺癌细胞NCI-H460为供试细胞株,采用SRB法测试化合物的细胞毒活性,以金黄色葡萄球菌(SA)、大肠杆菌(EC)和铜绿假单胞菌(PA)为指示菌株,采用改良的MMT法测试化合物的抗菌活性。结果:从发酵物中分离并鉴定了7个化合物,分别为:2,4-二羟基-3,5,6三甲基苯甲酸甲酯(1)、对羟基苯乙酮(2)、5-羟甲基糠酸(3)、isochromophilone VIII(4)、麦角甾醇(5)、过氧化麦角甾醇(6)、啤酒甾醇(7)。结论:化合物1为首次从青霉属真菌中分离得到,化合物3具有较强的抗菌活性,化合物4具有显著的胞毒活性。

Study on Secondary Metabolites of Marine Fungus Penicillium sp. FS60 from the South China Sea

Objective:To study the secondary metabolites of the marine fungus Penicillium sp.FS60 from the South China Sea and their cytotoxicities.Methods:The compounds were isolated from the culture of strain FS60 by various chromatographic methods(silica gel,reverse silica gel,Sephadex-LH20,preparative TLC,HPLC and PTLC)and recrystallization.Their structures were identified by extensive analysis of their spectroscopic data.Compounds were tested for their cytotoxicities against SF-268,MCF-7,and NCI-H460 cell lines by SRB method.While,Compounds were tested for their antibacterial activities against S.aureus,E.coli and P.aeruginosa.Results:Seven compounds were isolated from the culture and identified as methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate(1),4-hydroxyacetophenone(2),5-hydroxymethyl-furoic acid(3),isochromophilonesⅧ(4),ergosterol(5),ergosterol peroxide(6),and cerevisterol(7).Conclusion:Compound 1 is isolated from the genus Penicillium for the first time.Compound 3 is demonstrated to have significant inhibition against S.aureus and P.aeruginosa.Compound 4 is demonstrated to have significant inhibition against the three cell lines.