| 脐带血间充质干细胞移植治疗缺氧缺血性脑损伤新生大鼠的疗效 |
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| 引用本文： | 李湘云,胡博,李建斌,单泓,赵林娜,段艳丽,韩小改,杨鹏辉.脐带血间充质干细胞移植治疗缺氧缺血性脑损伤新生大鼠的疗效[J].实用儿科临床杂志,2010(8). |
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| 作者姓名： | 李湘云 胡博 李建斌 单泓 赵林娜 段艳丽 韩小改 杨鹏辉 |
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| 作者单位： | 郑州大学第三附属医院脑瘫康复中心;河南省红十字血液中心; |
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| 摘 要： | 目的探讨混合培养的人脐血间充质干细胞(MSCs)对缺氧缺血性脑损伤(HIBD)新生大鼠的疗效。方法无菌条件下收集20份健康足月新生儿脐血,采用密度梯度离心法分离出脐血单个核细胞,再通过贴壁细胞培养法培养出人脐血MSCs。当细胞传至第3代时,随机取培养的8份脐血MSCs(半量)为A组,8份脐血MSCs(半量)为B组,将A、B组剩余的半量脐血MSCs混合为C组,3组细胞密度均为1×106L-1。混合培养的第3代脐血MSCs用流式细胞仪检测细胞表面抗原CD29、CD34、CD44、CD105的表达。选择P4代人脐血MSCs作为移植细胞,并经5-溴-2-尿嘧啶脱氧核苷(BrdU)体外标记。7日龄SD大鼠45只制备HIBD模型,造模中死亡3只,余42只分为混合移植组(n=16)、单份移植组(n=16)和对照组(n=10)。造模第3天,在立体定位条件下,移植组大鼠经左侧大脑皮质注入人脐血MSCs,对照组在相同部位注入相同体积的PBS。在细胞移植第7天,随机选择6只移植组大鼠处死取脑,采用免疫组织化学方法观察移植细胞在脑内的存活、迁移和分化情况。移植第1、7、14、21、28天采用改良神经功能损害评分(mNSS)对3组大鼠...
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| 关 键 词： | 脐血 间充质干细胞 混合培养 缺氧缺血性脑损伤 移植 分化 |
Effect of Umbilical Cord Blood Mesenchymal Stem Cell Seeding Treatment for Neonatal Rats with Hypoxic-Ischemic Brain Damage |
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| LI Xiang-yun,HU Bo,LI Jian-bin,SHAN Hong,ZHAO Lin-na,DUAN Yan-li,HAN Xiao-gai,YANG Peng-hui.Effect of Umbilical Cord Blood Mesenchymal Stem Cell Seeding Treatment for Neonatal Rats with Hypoxic-Ischemic Brain Damage[J].Journal of Applied Clinical Pediatrics,2010(8). |
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| Authors: | LI Xiang-yun HU Bo LI Jian-bin SHAN Hong ZHAO Lin-na DUAN Yan-li HAN Xiao-gai YANG Peng-hui |
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| Institution: | LI Xiang-yun1,HU Bo1,LI Jian-bin2,SHAN Hong2,ZHAO Lin-na2,DUAN Yan-li2,HAN Xiao-gai2,YANG Peng-hui2(1.Center for Cerebral Palsy Rehabilitation,the Third Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,Henan Province,China,2.Red Cross Blood Center in Henan Province,China) |
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| Abstract: | Objective To assess the effect of human umbilical cord blood mesenchymal stem cells (MSCs) by mixed culture of intracerebral transplantation in the neonatal rats with hypoxic-ischemic brain damage(HIBD).Methods Under sterile conditions,the cord blood of 20 healthy full-term neonates were collected by density gradient centrifugation to isolate mononuclear cells from cord blood and then by adherent cells to develop human umbilical cord blood MSCs.When the cells were in the 3rd generation,8 parts of the cultur... |
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| Keywords: | umbilical cord blood mesenchymal stem cell mixed culture hypoxic-ischemic brain damage transplantation differentiation |
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