| 重楼醇提物对非小细胞肺癌细胞活性影响的实验研究 |
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| 引用本文： | 付艳丽,林燕,段春燕,王巧琳.重楼醇提物对非小细胞肺癌细胞活性影响的实验研究[J].上海中医药大学学报,2017,31(2):57-61. |
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| 作者姓名： | 付艳丽 林燕 段春燕 王巧琳 |
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| 作者单位： | 新疆医科大学附属中医医院肿瘤一科新疆乌鲁木齐830000,新疆医科大学附属中医医院肿瘤一科新疆乌鲁木齐830000,新疆医科大学附属中医医院肿瘤一科新疆乌鲁木齐830000,新疆医科大学附属中医医院肿瘤一科新疆乌鲁木齐830000 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金资助项目(2016DOIC136) |
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| 摘 要： | 目的:观察中药重楼醇提物对非小细胞肺癌细胞活性的影响。方法:将人大细胞肺癌NCIH460细胞株、人肺腺癌A549细胞株悬液分别接种于96孔细胞培养板中,24 h细胞贴壁后依次加入不同浓度的含重楼醇提物培养液进行干预,并设RPMI-1640培养液为空白对照组。药物干预48 h后,采用MTT法测定不同浓度药物对NCI-H460、A549细胞增殖的影响,使用Hoechst荧光染色法检测对细胞凋亡的影响。结果:与空白对照组比较,重楼醇提物在不同浓度下对NCI-H460和A549两株肺癌细胞的生长均有抑制作用(P0.05~0.001),且呈浓度依赖性;对两种细胞的半数抑制浓度(IC_(50))分别为30μg/ml和15μg/ml。不同浓度的重楼醇提物作用于NCI-H460、A549细胞后,随着药物浓度的增加,凋亡细胞数亦明显增加,并伴随出现细胞的坏死、轮廓不清,甚至细胞解体等。结论:重楼醇提物可明显降低NCI-H460和A549细胞的活性并促进其凋亡,且呈剂量依赖性。
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| 关 键 词： | 重楼 非小细胞肺癌 细胞活性 体外实验 |
| 收稿时间： | 2016/12/6 0:00:00 |
Experimental Study on the Effects of Alcohol Extract of Rhizoma Paridis on Cell Activity in Non-small Cell Lung Cancer |
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| FU Yan-li,LIN Yan,DUAN Chun-yan and WANG Qiao-lin.Experimental Study on the Effects of Alcohol Extract of Rhizoma Paridis on Cell Activity in Non-small Cell Lung Cancer[J].Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai,2017,31(2):57-61. |
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| Authors: | FU Yan-li LIN Yan DUAN Chun-yan and WANG Qiao-lin |
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| Institution: | The First Department of Oncology,Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumchi,830000,The First Department of Oncology,Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumchi,830000,The First Department of Oncology,Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumchi,830000 and The First Department of Oncology,Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University,Urumchi,830000 |
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