| 90Y标记单克隆抗体CD45的研究 |
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| 引用本文： | 扶云碧,李贵平,孟凡义.90Y标记单克隆抗体CD45的研究[J].南方医科大学学报,2006,26(8):1230-1232. |
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| 作者姓名： | 扶云碧 李贵平 孟凡义 |
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| 作者单位： | 南方医科大学南方医院,血液科,广东,广州,510515;南方医科大学南方医院,核医学科,广东,广州,510515 |
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| 基金项目： | 广东省自然科学基金;广东省广州市科技计划 |
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| 摘 要： | 目的 制备^90Y标记的CD45单克隆抗体，为用^90Y-CDTPA-CD45mAb治疗急性白血病奠定基础。方法 利用环酐DTPA为络合剂，螯合^90Y与CD45mAb，并对^90Y-CDTPA-CD45mAb的标记率、放化纯度、稳定性、比活度、免疫活性进行检测。结果 在CDTPA与IgG的最佳摩尔比为20：1的条件下，^90Y-CDTPA-CD45mAb的标记率为95％，放化纯度达99．8％，稳定性良好，比活度为1.9mCi／mg，免疫活性与未标记的CD45mAb相比无明显差异。结论 ^90Y-CDTPA-CD45mAb是一个较理想的靶向治疗制剂．可以用于下一步的急性白血病治疗研究。
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| 关 键 词： | 90Y CD45单抗 环酐DTPA |
| 文章编号： | 1673-4254（2006）08-1230-03 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2005年12月20 |
Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90 |
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| FU Yun-bi,LI Gui-ping,MENG Fan-Yi.Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90[J].Journal of Southern Medical University,2006,26(8):1230-1232. |
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| Authors: | FU Yun-bi LI Gui-ping MENG Fan-Yi |
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| Institution: | Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. hnfyb@yahoo.com.cn |
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| Abstract: | OBJECTIVE: To explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy. METHODS: CD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected. RESULTS: With the optimal molar ratio of CDTPA/Ab at 20:1, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb. CONCLUSION: (90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia. |
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| Keywords: | 90Y |
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