| 病毒与人类基因组同源性初探 |
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| 引用本文： | 常雅萍,马晶,张猛,于永利. 病毒与人类基因组同源性初探[J]. 吉林大学学报(医学版), 1997, 0(3) |
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| 作者姓名： | 常雅萍 马晶 张猛 于永利 |
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| 作者单位： | 基础医学院免疫学教研室 |
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| 摘 要： | 探讨病毒与人类基因组同源性问题。选用ＥＢ病毒（ＥＢＶ），肠道病毒（ＥＶｓ）、单纯疱疹病毒－１型（ＨＳＶ－１），丙型肝炎病毒（ＨＣＶ）特异性引物，以６份健康成人外周血ＤＮＡ为模板，分别进行聚合酶链式反应（ＰＣＲ）。初步结果表明：有５份ＤＮＡ样品可通过ＥＢＶ、ＨＣＶ特异性引物扩增。ＥＢＶ引物扩增后的片段大小在１５４～２３４ｂｐ之间；ＨＣＶ引物扩增后的片段大小约２００ｂｐ和１２０ｂｐ。提示病毒某些基因可能存在人类基因组中或两者具有很高的同源性。
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| 关 键 词： | 病毒基因 人类基因组 聚合酶链反应 同源性 |
Preliminary study on genome homology of viruses and human |
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| Chang Yaping,Ma Jing,Zhang Meng,et al. Preliminary study on genome homology of viruses and human[J]. Journal of Jilin University: Med Ed, 1997, 0(3) |
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| Authors: | Chang Yaping Ma Jing Zhang Meng et al |
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| Affiliation: | Department of Immunology |
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| Abstract: | Human genomic DNAs isolated from peripheral blood of six healthy adults were amplified in a polymerase chain reaction (PCR) by using EBV ,EVsHSV-1,HCV specific primers individually.The preliminary results showed that five of genomic DNAs were between 154～234 bp by using EBV specific primers ,and the size of amplificed products were about 200 bp and 120 bp,suggesting that the viral genomic fragments either harbor in or share high homology with human genome. |
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| Keywords: | Viral genome Human genome Polymerase chain reaction Homology |
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