Multiplex PCR for typing strains of Toxoplasma gondii |
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Authors: | Ajzenberg Daniel Dumètre Aurélien Dardé Marie-Laure |
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Affiliation: | Laboratoire de Parasitology-Mycologie, Faculté de Médecine EA 3174, Limoges, France. |
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Abstract: | A multiplex PCR assay was designed for multilocus strain typing of Toxoplasma gondii based on length polymorphism of five microsatellite markers. Eight T. gondii strains already sequenced at these five markers were used as control isolates. This method is simple, rapid, reproducible, and adapted to a large set of isolates. |
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