非浓缩尿蛋白电泳在1型糖尿病肾病早期诊断中的应用

目的探讨非浓缩尿蛋白电泳在1型糖尿病肾病(DM)早期诊断中的意义。方法采用十二烷基黄酸钠-琼脂糖凝胶电泳法(SDS-AGE)对1型糖尿病(DM)病人47例和健康者30例行晨尿电泳,根据DM患者尿清蛋白排泄率分为DM组和DN组。结果DN组与DM组及对照组比较,清蛋白条带深且宽,DN组清蛋白条带阳性率较后两者有显著性差异(P< 0.01)。DM组和对照组比较无显著性差异。DN组可见大或小分子条带,DM组和对照组未见大或小分子条带。结论DM病人尿蛋白电泳出现较正常人深且宽的清蛋白条带,或同时出现大、小分子蛋白条带,病人进入早期DN的可能性很大。SDS- AGE可作为诊断早期DN的辅助检查手段。实用儿科临床杂志,2005,20(5):399—400

Application of Non-Concentrated Proteinuria Electrophroresis in Early Diagnosis of Type 1 Diabetic Nephropathy

Objective To investigate the significance of non - concentrated proteinuria electrophroresis in early diagnosis of type 1 diabetic nephropathy(DN). Methods A total of 47 selected patients with type 1 diabetes and 30 healthy controls were recruited in the study. Fourty seven patients were divided into diabetes mellitus(DM) group and DN group based on the rate of urinary excretion of albumin. Sodium dodcyl sulfate - agarose gel electrophoresis(SDS - AGE) was used to analyze the proteinuria in patients and controls group. Results The positive ratio of urinary albumin SDS - AGE was significantly higher in DN group than in DM group and normal control(group P<0.01), and urinary albimin was fuscously and widely in DN group. Conclusion The urinary protein SDS-AGE can be considered a indicator in early diagnosis of type 1 diabetic nephropathy. J Appl Clin Pediatr, 2005,20(5):399-400