兔油酸型呼吸窘迫综合征TXB_2和PGI_2的变化及相互关系

本文对血栓素、前列腺环素在实验性呼吸窘迫综合征中的变化和相互关系进行了研究,并比较了肺动脉、左心房血浆和肺组织中的含量变化。实验分两组进行,Ⅰ组盐水对照,Ⅱ组实验组。动物注射油酸(0.045ml/kg)后,血浆及肺组织中的TXB_2(TXA_2的稳定代谢产物)立即升高,6-酮-PGF_1α(PGI_2的稳定代谢产物)则较晚上升,TBX_2/6-酮-PGF_1α先上升,后下降。结果提示:(1).TXA_2和PGI_2在油酸ARDS模型中是显著增加的;(2)血浆中与肺组织中的值有时不一致;(3)TXA_2/6-酮-PGF_1α在ARDS的发病机理中可能有重要作用。

Interrelationship between the Contents of Thromboxane and Prostacyclin in Plasma and Lung Tissue in ARDS in Rabbits

This study was designed to investigate the changes of the contents of throm-boxane (TXA2) and prostacyclin (PGI2) in the pulmonary tissue and in the plasma of the pulmonary arteries and left atrium of the rabbits during ARDS induced with oleic acid injection and the interrelationship between the two substances.The contents of TXA2 and PGI2 were determined indirectly,by measuring the amounts of TXB2 and 6-keto-PGF1a,the stable metabolites of TXA2 and PGI2 respectively,with radioimmunoassay.After an injection of oleic acid to the rabbits (0.045ml/kg),the contents of TXB2 in both the plasma and lung tissue were significantly elevated The content of 6-keto-PGF1a in the plasma significantly increased at 30 minutes and reached the highest point at 180 minutes after the injection,and that in the lung tissue began to increase at 5 minutes and reached the peak at 60 minutes after injection The ratio of TXB2/6-keto-PGI18 increased in the early stage after injection and then reduced both in the plasma and the lung tissue.The results indicate that the contents of TXA2 and PGI2 in the plasma are not always parallel with those in the lung tissue,which suggests that the variation of the ratio of TXA2/PGI2 may play an important role in the pathogenesis of ARDS.