非小细胞肺癌中表皮生长因子受体基因拷贝的检测及其临床意义

目的 检测国人非小细胞肺癌标本中表皮生长因子受体(EGFR)基因高拷贝的发生率及与临床特征的关系.方法 236例非小细胞肺癌标本制作成组织微阵列,使用Vysis公司的荧光原位杂交探针检测EGFR基因拷贝数.结果 193例标本得到结果 ,检测到EGFR基因高拷贝81例(占42.0%),其中42例(21.8%)为高多倍体,39例(20.2%)为基因扩增.其发生与病理、分期、性别、吸烟等临床特征无明显相关,与患者的1-3年生存率无明显相关.结论 国人肺癌中EGFR基因拷贝数的分布情况与西方人类似,与临床特征及预后无明显相关.

Epidermal growth factor receptor gene copy number in Chinese patients with non-small cell lung cancer and clinical significance

Objective To evaluate the incidence of epidermal growth factor receptor (EGFR)gene high copy in Chinese patients with non-small-cell lung cancer (NSCLC) and its relation with clinical features. Methods 236 surgically resected NSCLC samples constructed into tissue micro-array were investigated for EGFR gene copy number by fluorescence in situ hybridization (FISH) with commercial probe from Vysis. Results Of the 263 samples tested, 193 samples were informative. Eighty-one samples(42.0%) showed EGFR gene high copy number, including 42 (21.8%) cases of high polysomy and 39(20.2%) cases of gene amplification. Statistical analysis revealed that there was no any association of gent high copy number with clinical parameters including histopathology,TNM stage, sex,smoking history and 1-3 year survival rate. Canclusion NSCLC samples from Chinese patients with NSCLC had similar EGFR gene copy number revealed by FISH with that from Caucasian patients.