| dbcAMP诱导的EoL-1分化和凋亡与bcl-2家族的关系 |
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| 引用本文： | 台桂香, 刘静, 高素君. dbcAMP诱导的EoL-1分化和凋亡与bcl-2家族的关系[J]. 中国肿瘤临床, 2004, 31(13): 729-731. |
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| 作者姓名： | 台桂香 刘静 高素君 |
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| 作者单位： | 1.吉林大学基础医学院免疫教研室, 长春市 1300212;;2.长春市儿童医院;;3.吉林大学第一医院血液科 |
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| 基金项目： | 教育部留学回国人员科研启动基金 |
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| 摘 要： | 目的:探讨dbcAMP(dbutyryl cyclic AMP)诱导的EoL-1(eosinophilic leukemia cell line)分化和凋亡与bcl-2家族的关系。方法:通过免疫印记和流式细胞仪调查bcl-2家族蛋白在dbcAMP刺激的EoL-1细胞中的表达。结果:EoL-1在正常培养条件下表达高水平的bcl-2、bcl-xL和中等水平的bax,但未表达bcl-xs。dbcAMP诱导了EoL-1细胞下调表达bcl-2和bax及上调表达bcl-xs。结论:dbcAMP诱导EoL-1分化和凋亡与bcl-2的降低以及bcl-xs的升高有关.
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| 关 键 词： | dbcAMP bcl-2家族 EoL-1凋亡 |
| 文章编号： | 1000-8179(2004)13-0729-03 |
| 收稿时间： | 2003-06-02 |
| 修稿时间： | 2003-11-11 |
The Relationship between the Differentiation and Apoptosis in EoL-1 Induced by dbcAMP and the bcl-2 Family |
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| Tai Gui-xiang, Liu Jing, Gao Su-jun. The Relationship between the Differentiation and Apoptosis in EoL-1 Induced by dbcAMP and the bcl-2 Family[J]. CHINESE JOURNAL OF CLINICAL ONCOLOGY, 2004, 31(13): 729-731. |
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| Authors: | Tai Gui-xiang Liu Jing Gao Su-jun |
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| Affiliation: | 1.Department of Immunology, Basic Medical Institute of Jilin University, Changchun |
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| Abstract: | Objective: To research the relationship between differentiation and apoptosis of EoL-1 induced by dbcAMP and bcl-2 family. Methods: We examined the bcl-2 family expression in EoL-1 cells cultured with dbcAMP by Western blot and flow cytometry. Results: We found that EoL-1cells under normal culture conditions expressed bcl-2, bcl-xL and bax at high level but not bcl-xs. dbcAMP downregulated bcl-2 and bax level but upregulated bcl-xs level. Conclusion: It surggests that the eosinophilic differentiation and apoptois in EoL-1 stimulated by dbcAMP partly have relations with the decrease of bcl-2 and increase of bcl-xs. |
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| Keywords: | dbcAMP EoL-1 apoptosis bcl-2 family |
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