| 外环境中脊髓灰质炎病毒分离的实验研究 |
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| 引用本文： | 丁昌慧,邵荣标,钱勣虎,严建国,汤权,王秀娟,刘玲,吴巨飞.外环境中脊髓灰质炎病毒分离的实验研究[J].中国卫生检验杂志,2000,10(2):163-165. |
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| 作者姓名： | 丁昌慧 邵荣标 钱勣虎 严建国 汤权 王秀娟 刘玲 吴巨飞 |
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| 作者单位： | 1. 江苏省盐城市卫生防疫站,盐城,224002
2. 江苏省南通医学院微生物学教研室 |
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| 摘 要： | 目的 建立一种简便，实用的检测大量水样中的微量脊髓灰质炎病毒的方法。方法 将Ｌ２０Ｂ细胞加入大量的含微量脊髓灰质炎病毒的水中，充分作用后收集细胞，作病毒分离培养，同时以ＮａＣｌ－ＡｌＣｌ３沉淀法作对照。结果 １０００ｍｌ水样中含病毒１００、１０ＴＣＤ５０时能全部检出，当含１￣２ＴＣＤ５０时１４份样本可检出１３份。ＮａＣｌ－ＡｌＣｌ３沉淀法在１００ＴＣＤ５０时４份样本中仅检出１份，其它各浓度的４份样
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| 关 键 词： | 水 脊髓灰质炎病毒 浓集 L20B细胞 吸附 |
| 文章编号： | 1004-8685(2000)02-0163-03 |
| 修稿时间： | 1999年1月6日 |
The Experimental Study on Culturing Poliovirus in Environmental Water |
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| Ding Changhui, Shao Rongbiao, Qian Jihu,et al..The Experimental Study on Culturing Poliovirus in Environmental Water[J].Chinses Journal of Health Laboratory Technology,2000,10(2):163-165. |
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| Authors: | Ding Changhui Shao Rongbiao Qian Jihu |
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| Institution: | Ding Changhui, Shao Rongbiao, Qian Jihu, et al. |
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| Abstract: | Objective ]: To set up a simple, practical, new method for detecting minimum polio virus in large volume water. Method ]: Adding L20B cells into the large volume water containing minimum polio viruses. Collecting the cells after they fully reacted with viruses, then culturing viruses, with the method of NaC1-AIC1_3, sedimentation as control. Results ]: If the amount of viruses was 100, 10TCD_50,, per 1000ml water, the viruses could be detected. When the amount was l~ZTCD_50, the viruses in 13 of 14 samples can be detected. While the viruses of 100TCD_50 per 1000ml water in 1 of 4 samples can be detected with the method of NaCI--A1C1_3, sedimentation, but the viruses of other concentrations in all samples couldn't be detected. |
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| Keywords: | Water Poliovirus Concentration L20B cell Absorption |
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