曲张大隐静脉管壁细胞凋亡水平的变化

目的:观察不同部位曲张大隐静脉管壁细胞的凋亡水平的变化.方法:收集21例曲张大隐静脉(观察组)与12例正常大隐静脉(对照组),分别采用TUNEL法和免疫荧光法检测各自上、中、下3段管壁的细胞凋亡情况.结果:观察组管壁(内膜和中膜)偶见单个凋亡细胞,且3段分布基本相同,对照组管壁(内膜和中膜)各段则见较多凋亡细胞,且上段较下段更为明显.定量分析显示,观察组各段的内膜或中膜的细胞凋亡率均明显低于对照组的相应区域(均P＜0.05)；两组组内各段间细胞凋亡率比较,部分有统计学差异(P＜0.01或P＜0.05),总体趋势,上段高于下段,内膜高于中膜.结论:曲张大隐静脉管壁细胞凋亡明显下调,且无正常大隐静脉管壁凋亡水平的节段间差异.

Alterations of apoptotic level in wall of great saphenous varicose vein

Objective: To observe the apoptotic level alterations in the wall of different segments of great saphenous varicose vein. Methods: Twenty great saphenous varicose veins (observation group) and 12 normal great saphenous veins (control group) were collected, and the apoptosis in their upper, middle and lower segments were determined by TUNEL assay and immunofluorescence staining, respectively. Results: In observation group, only scattered apoptotic cells were found in the venous walls (tunica intima and tunica media), which were almost uniformly distributed at the three segments, while there were considerably more apoptotic cells in the venous walls (tunica intima and tunica media) in control group, and the apoptosis was more evident in the upper segments than those of the lower segments. The quantitative analysis showed that the apoptotic rates in either the tunica intima or tunica media in observation group were significantly lower than those in their corresponding regions in control group (all P<0.05); statistical significances in apoptotic rate were found between some of the segments after intra-group comparison for each group (P<0.01 or P<0.05), but generally, the apoptotic rate was higher in the upper segment than that in the lower segment and higher in the tunica intima than that in the tunica media. Conclusion: In the wall of great saphenous varicose vein, the apoptotic level is decreased, and there was no apoptotic rate difference between segments in the normal great saphenous vein.