microRNA-497 在肝细胞癌中的表达及意义

目的:探讨microRNA-497(miR-497)在肝细胞癌组织中的表达及其意义.方法:收集40例肝细胞癌及对应癌旁组织标本,用qRT-PCR方法检测标本中miR-497的表达；人肝癌SMMC-7721细胞经miR-497模拟物转染后,用MTT和流式细胞术检测其增殖能力与凋亡水平的变化,并用qRT-PCR和Western blot检测miR-497潜在靶点Bcl-w的mRNA与蛋白的表达.结果:miR-497在肝癌组织中的表达水平明显低于其癌旁组织(1.181±0.779)vs.(14.599±5.266),P＜0.05]；转染miR-497模拟物的SMMC-7721细胞增殖能力明显降低,细胞凋亡增加,Bcl-w的mRNA与蛋白表达均明显降低(与未转染的SMMC-7721细胞比较,均P＜0.05).结论:肝癌组织中miR-497表达下调,该下调可能导致靶基因Bcl-w表达升高,从而促进肿瘤的发生与发展.

MicroRNA-497 expression in hepatocellular carcinoma and its significance

Objective: To investigate the microRNA-497(miR-497) expression in hepatocellular carcinoma (HCC) tissues and its significance. Methods: Forty HCC and their adjacent tissue specimens were collected, and the miR-497 expression in these specimens were measured by qRT-PCR method. Human HCC SMMC-7721 cells were transfected with miR-497 mimic, and then their proliferation ability and apoptosis level were determined by MTT assay and flow cytometry technique, and the mRNA and protein expressions of Bcl-w as a potential target of miR-497 were detected by qRT-PCR and Western blot analysis, respectively. Results: The expression level of miR-497 in HCC tissues was significantly lower than that in the adjacent tissues (1.181±0.779 vs. 14.599±5.266, P<0.05). In SMMC-7721 cells transfected with miR-497 mimic, the cell proliferation was decreased while cell apoptosis was increased, and both mRNA and protein expressions of Bcl-w were reduced significantly (all P<0.05 vs. untransfected SMMC-7721 cells). Conclusion: The miR-497 expression is down-regulated in HCC tissues, which may probably result in the increased expression of its target gene Bcl-w and then promote the occurrence and development of the tumor.