bFGF预处理对Iso致大鼠心肌损伤及Fas/FasL系统的影响

目的：研究碱性成纤维细胞生长因子(bFGF)对异丙基肾上腺素(Isoprenaline，Iso)致大鼠心肌损伤心肌细胞凋亡及Fas／FasL系统的影响。方法：将Wistar雄性大白鼠随机分成三组：对照组、Iso损伤纽、bFGF预处理组，光镜观察心肌组织的病理变化，TUNEL法检测心肌细胞凋亡，免疫组化和原位杂交方法检测Fas、FasL、Caspase-3蛋白及mRNA的表达水平。结果：Iso损伤组心肌坏死较重，有显著心肌细胞凋亡，且Fas、FasL、Caspase-3基因mRNA及蛋白表达水平明显上调，bFGF预处理组心肌坏死明显减轻，凋亡细胞数减少，Fas、FasL、Caspase-3基因mRNA及蛋白表达水平明显下调。结论：bFGF可通过抑制Fas、FasL、Caspase-3的蛋白及mRNA表达，减少心肌细胞凋亡而减轻保护大鼠的心肌损伤。

THE ROLE OF bFGF ON THE MYOCARDIAL INJURY AND APOPTOSIS INDUCED BY ISOPRENALINE AND CORRELATED GENES EXPRESSION

Objective: To study the role of bFGF on the myocardial injury and apoptosis induced by Iso and correlated genes expression. Methods: Wistar rats were divided into three groups at random. They were the control group, the Iso group and the bFGF group respectively. The pathological changes were observed by microscope. The apoptosis were observed by TUNEL. The mRNA and protein expression of Fas, FasL, Caspase-3 were tested with immunohistochemistry and in situ hybridization methods. Results: In the Iso group, the myocardial necrosis were more serious, and there were more apoptosis. Moreover the expression level of mRNA and protein of Fas, FasL, Caspase-3 increased. In the bFGF group, the myocardial necrosis were relieved and there were less apoptosis. The expression level of mRNA and protein of Fas, FasL, Caspase-3 were decreased clearly. Conclusion: The bFGF can protect rat myocardium from injury by inhibiting the expression of Fas, FasL, Caspase-3 increasing myocardial apoptosis.