Strongyloides stercoralis: identification of antigens in natural human infections from endemic areas of the United States |
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| Authors: | Afzal A Siddiqui Nora M Koenig Michael Sinensky Steven L Berk |
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| Institution: | (1) Department of Biochemistry and Molecular Biology, Box 70581, James H. Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA Tel: (423) 439-8347/439-8353; Fax: (423) 439-8366; e-mail: siddiqui@access.etsu-tn.edu, US;(2) Department of Internal Medicine James H. Quillen College of Medicine East Tennessee State University Johnson City, TN, USA, US |
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| Abstract: | Using Western-blot analysis, we identified eight immunodominant antigens (apparent molecular weights 96, 86, 75, 56, 41,
32, 28, and 26 kDa) of Strongyloides stercoralis in natural human infections. For this study, 78 individual serum samples were obtained from S.␣stercoralis-infected patients residing in endemic areas of the United States. Poly A+ RNA was translated in vitro in the rabbit-reticulocyte lysate system. The newly synthesized translation products were immuno-precipitated
with S. stercoralis human infection sera. All eight of the identified antigens were detected in the immunoprecipitates. The potential of these
antigens as targets for immunodiagnosis is also discussed.
Received: 28 January 1997 / Accepted: 25 April 1997 |
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