口服高分子微球疫苗的制备研究

 目的：研制口服靶向长效的包载抗原蛋白的疫苗微球。方法：以可生物降解高分子聚-D，L-乳酸-聚乙二醇(PLA-PEG)为基质材料。采用复合乳液溶剂蒸发技术包载外膜蛋白(OMP)及人血清白蛋白(HSA)，制备出粒径小于2 μm的微球。考察了制备工艺对微球粒径及蛋白含量的影响。结果：采用适当配比的聚乙烯醇(PVA)水溶液为分散介质，可得到期望粒径的PLA-PEG微球。对抗原蛋白的包裹量可达1%左右，并视蛋白性质而定。表面活性剂可改善微球对蛋白的包裹条件。结论：可生物降解的PLA-PEG能对抗原蛋白进行有效包裹，并制得粒径小于2 μm的微球。

Preparation of oral polymeric microspheres carrying vaccine

OBJECTIVE:To prepare oral biodegradable polymeric microspheres with micron size carrying antigen protein outer membrane protein(OMP) for an effective immunization.METHOD:By composite emulsion solvent evaporation technique with biodegradable poly-D,L-lactide-polyethylene glycol(PLA-PEG) as matrix microspheres with sizes in 0.1～2 μm encapsulating antigen protein OMP was prepared.Human serum albumin(HSA) as a model protein was also encapsulated in the microspheres to investigate the conditions of protein encapsulation.Differential scanning calorimetry(DSC) was used for estimating the internal crystallinity of PLA-PEG microspheres.UV absorbance at 283 nm was used for quantitating the amount of protein in PLA-PEG microspheres.RESULTS:The aim microspheres were obtained.The OMP can be effectively encapsulated in the PLA-PEG microsp-heres.The nature of protein would affect the amount of protein in the microspheres.CONCLUSION:Polyvinyl alcohol(PVA) with 88% hydralyzation was suitable for preparing PLA-PEG microspheres carrying antigen protein.The amount of antigen protein in the microspheres could be over 1% which was able to be used as oral vaccine microspheres.