D-赖氨酸、L-谷氨酰胺和L-酪氨酸对格列美脲及其顺式异构体识别的电喷雾质谱法研究

目的 使用电喷雾质谱技术，研究3种氨基酸（D-赖氨酸、L-谷氨酰胺和L-酪氨酸）作为手性选择探针对格列美脲和格列美脲异构体的识别效应。方法 分别将3种氨基酸与格列美脲及其顺式异构体溶液混合，用电喷雾/离子阱质谱提取非共价结合的复合物，在二级质谱中通过碰撞诱导解离（CID）方法给予不同的碎裂能量，考察碎片离子的丰度从而进行格列美脲及其顺式异构体的识别。结果 L-酪氨酸、D-赖氨酸和L-谷氨酰胺对格列美脲及其顺式异构体均有识别效应，识别率分别为1.61、2.92和2.17。结论 以3种手性氨基酸作为立体异构区分探针，首次实现了格列美脲和格列美脲顺式异构体的质谱法快速识别。

Study on chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine, L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry

Objective Chiral recognition for the glimepiride and glimepiride-cis-isomer by applying three amino acid (D-Lysine, L-Glutamine and L-Tyrosine) as chiral probes based on electrospray ionization mass spectrometry (ESI-MS) was achieved. Methods glimepiride/glimepiride-cis-isomer solutions were mixed with three amino acid solutions. The complex was extracted by ESI-MS and then the fragmentation abundance was investigated applying collision induced dissociation (CID) by MS/MS, which is the basis of chiral recognition for glimepiride and glimepiride-cis-isomer. Results Chiral recognition effect was achieved with the recognition rate (R) 1.61, 2.92 and 2.17 for D-Lysine, L-Glutamine and L-Tyrosine respectively.Conclusion 3 kinds of chiral amino acids were used as probes to distinguish between stereoisomers, and rapid identification of glimepiride and glimepiride cis isomer by mass spectrometry come true for the first time.