水飞蓟宾对高脂诱导非酒精性脂肪肝大鼠的调脂保肝作用

目的 探讨水飞蓟宾对高脂诱导的非酒精性脂肪肝（NAFL）模型大鼠的调脂保肝作用。方法 采用ig高脂乳剂配合高脂饲料制备大鼠NAFL模型，持续4周，对照组给予生理盐水和普通饲料。模型大鼠随机分为模型组、辛伐他汀（阳性药，1.8 mg/kg）组和水飞蓟宾低、中、高剂量（18.9、37.8、75.6 mg/kg）组，第5周在继续造模的基础上ig给药，每天1次，持续8周。末次给药后，称取肝脏质量并计算肝系数；HE染色观察肝组织病理形态；腹主动脉取血，分离血清，试剂盒法检测三酰甘油（TG）、总胆固醇（TC）、高密度脂蛋白（HDL）、低密度脂蛋白（LDL）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）水平。结果 连续治疗8周后，与对照组比较，水飞蓟宾高、中剂量均可明显改善肝组织脂肪变性程度；各剂量组肝系数均显著下降（P<0.05、0.01）；各剂量组均显著降低血清TC、TG、AST、ALT水平（P<0.05、0.01）；高、中剂量组显著降低LDL、升高HDL水平（P<0.01）。结论 水飞蓟宾对NAFL大鼠发挥治疗作用，其作用可能与降脂、保肝有关。

Lipid hepatoprotective effect of silibinin on fat-induced nonalcoholic fatty liver ratmodel

Objective To investigate the lipid hepatoprotective effect of silibinin on high fat diet-induced nonalcoholic fatty liver (NAFL) rat model and provide a theoretical basis for the treatment of silibinin on NAFL. Methods The NAFL rat model was established by administration of high fat emulsion and high fat diet. Rats in control group was treated with saline and normal diet.The model rats were randomly divided into model group, simvastatin (positive drug, 1.8 mg/kg) group,Silibinin groups with low, middle and high doses (18.9, 37.8 and 75.6 mg/kg). From the fifth week, NAFLrats were treated with different drugsonce a day for eight weeks. All rats were anaesthetized afterfinal administration, Livertissues were weighed for the calculation of hepatic coefficientThe hepatic morphology was observed through HE staining. Serum was obtained from abdominal aortic blood fordetection of triglyceride separation (TG), total cholesterol (TC), high density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate aminotransferase (AST),and alanine aminotransferase (ALT) levels.Results After eight-week treatment, compared with model group, middle and high doses of silibinincould significantly improve the hepatic steatosis. The levels of hepatic coefficient, serum TC, TG, AST and ALT in rats treated with individual dose of Silibinin were significantly decreased (P< 0.05, 0.01). Particularly, high dose of silibinin significantly reduced LDL level whereas elevated HDL level in serum (P< 0.01).Conclusion Silibinin has a therapeutic effect on nonalcoholic fatty liver rats, and possible mechanism is related to lipid-lowering and hepatic protection.