巴曲酶注射液对大鼠血栓栓塞性脑卒中急性超早期损伤的保护作用

目的 研究巴曲酶注射液对大鼠血栓栓塞性脑卒中急性超早期的保护作用。方法 自体血凝块闭塞左侧大脑中动脉法制备大鼠血栓栓塞性脑卒中模型，32只造模成功大鼠按神经缺陷程度随机分为4组：模型组及巴曲酶注射液低、高剂量（0.3、1.0 BU/kg）组和阿替普酶（rt-PA，9 mg/kg）组，每组8只，另设假手术组8只。造模1 h后尾iv给药，给药后6 h行神经功能评分，采用核磁共振（MIR）技术进行大鼠脑SE-T2WI序列扫描，测量脑病变范围；给药后24 h评分后取脑进行TTC染色，测量脑梗死范围；给药后6、24 h取血浆，测纤维蛋白原（FIB）浓度。结果 与模型组比较，巴曲酶注射液0.3 BU/kg治疗24 h（P<0.05）、1 BU/kg治疗6、24 h（P<0.05、0.01）显著改善大鼠神经功能评分；给药后6 h MRI结果显示，巴曲酶注射液0.3、1.0 BU/kg显著缩小病变范围（P<0.05、0.01）；给药后24 h TTC结果显示，巴曲酶注射液0.3、1.0 BU/kg显著缩小梗死范围（P<0.05）；巴曲酶注射液0.3、1.0 BU/kg于药后6、24 h均可显著降低血浆FIB浓度（P<0.05、0.01、0.001）。结论 巴曲酶注射液能改善大鼠脑缺血急性期受损神经功能、缩小脑病变范围、降低血浆FIB浓度，具脑保护作用。

Protection of Batroxobin Injection on super acute stage of thromboembolic cerebral stroke in rats

Objective To observe the effects of Batroxobin Injection on thromboembolic cerebral stroke by magnetic resonance imaging (MRI) and TTC staining.Methods Rat model of thromboembolic stroke was prepared after the left middle cerebral artery was occluded by autologous blood clots, and 32 rats with successful operation were divided into four groups according to the degree of neurological deficit: model group, Batroxobin Injection low and high dose (0.3, 1.0 BU/kg) group, and rt-PA (9 mg/kg) group, with eight rats in each group, and other eight rats in Sham group. Rats were administered 1 h after modeling by tail iv method. At 6 h after administration, neurological deficit score and MRI including SE-T2WI and DWI sequence scanning were measured. At 24 h after administration, the brain was cut for TTC staining to measure the infarct area, and blood FIB was measured.Results Compared with model group, Batroxobin Injection 0.3 BU/kg treatment for 24 h (P<0.05), 1 BU/kg treatment for 6 and 24 h (P<0.05, 0.01) could significntly improve the neurological function scores of rats. MRI results showed that Batroxobin Injection at dose of 0.3 and 1 BU/kg significantly reduced the lesion range (P<0.05 and 0.01). Results of TTC stain showed that Batroxobin Injection at dose of 0.3 and 1 BU/kg significantly reduced the infarct size (P<0.05). Batroxobin Injection at doses of 0.3 and 1 BU/kg can significantly lower plasma FIB concentration (P<0.05, 0.01, 0.001) 6 and 24 h after administration.Conclusion Batroxobin Injection can improve the damaged neural function, reduce scope of lesions, decrease plasma fibrinogen, with protective effects for cerebral ischemia in rats.