复方红景天对大鼠缺血性脑损伤后海马组织细胞凋亡的影响

[目的]探讨复方红景天对大鼠缺血性脑损伤后海马组织细胞凋亡的影响。[方法]80只雄性SD大鼠随机分为假手术组、模型组、尼莫地平(阳性对照)组与复方红景天高、低剂量组,线栓法制作大鼠大脑中动脉闭塞模型。复制模型前7 d灌胃给药,1次/日,连续10 d。测定大鼠行为学指标及脑梗死比例;分离海马组织,TUNEL法检测神经细胞凋亡,免疫印迹技术检测Bax、Bcl-2蛋白表达情况。[结果]与假手术组相比,模型组大鼠横木行走实验评分升高,前肢握力下降,脑梗死比例增大(P0.01);凋亡阳性细胞明显增多,Bax蛋白表达升高,Bcl-2蛋白表达降低(P0.01)。与模型组比较,复方红景天高、低剂量组均能降低大鼠横木行走实验评分、增加前肢握力,降低脑梗死的比例(P0.05);降低大鼠海马组织神经细胞凋亡率,降低促凋亡蛋白Bax的表达,升高抑凋亡蛋白Bcl-2的表达(P0.05)。[结论]复方红景天通过抑制海马组织神经细胞的凋亡,发挥对缺血性脑损伤的保护作用,相关机制与抑制线粒体凋亡途径有关。

Study on effect of Compound Rhodiola on apoptosis of hippocampus in rats with cerebral ischemia

[Objective] To study the effect of Compound Rhodiola on apoptosis of hippocampus cells after ischemic brain injury.[Methods] The 80 male SD rats were randomly divided into sham-operation group, model group, nimodiping group (positive control), and Compound Rhodiola high and low dose groups. Permanent models of middle cerebral artery occlusion by suture were made in rats. Drugs were given to the rats 7 d before establishment of the models, ig, once a day, for consecutive 10 d. The behavioral indexes were measured. The proportion of cerebral infarction was detected. Hippocampus tissue was separated, apoptosis rate of neurons was tested by using TUNEL method; the expression of Bcl-2 and Bax were observed by Western blot.[Results] Compared with sham-operation group, the scores of balance test of model group were increased, and grip strength was decreased and the proportion of cerebral infarction was increased (P<0.01). Apoptosis positive cells were evident increased. The expression of Bax was higher, but Bcl-2 was lower. Compared with model group, the scores of balance test of Compound Rhodiola high and low dose groups were decreased, and grip strength was increased and the proportion of cerebral infarction was decreased (P<0.01). Apoptosis positive cells were evident decreased. The expression of Bax was decreased, but Bcl-2 was increased.[Conclusion] The protection of Compound Rhodiola on ischemic brain injury through its inhibition of neuronal apoptosis, and the mechanism may be related with mitochondrial pathway.