Bullous pemphigoid IgG2 and IgG4 antibodies have a capability to inhibit a transient increase of intracellular Ca2+ induced by bullous pemphigoid IgG1 antibody. |
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| Authors: | M Suzuki S Harada H Yaoita H Nakagawa |
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| Affiliation: | Department of Dermatology, Jichi Medical School, Kawachi-gun, Tochigi, Japan. |
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| Abstract: | To elucidate the role of Bullous pemphigoid (BP) IgG subclasses in the transmembrane signal transduction of keratinocytes, we examined whether BP IgG2 or IgG4 inhibits the intracellular Ca2+ concentration ([Ca2+]i) increase induced by BP IgG1. IgG2 from one of five BP patients inhibited the increase of [Ca2+]i in human squamous cell carcinoma cell line; DJM-1 cells induced by BP IgG1. IgG4 from two of four BP patients inhibited the increase of [Ca2+]i induced by BP IgG1. In addition, a two fold quantity of IgG2 inhibited the increase of [Ca2+]i induced by BP IgG1. A two fold quantity of IgG4 from two of five BP patients inhibited the increase of [Ca2+]i induced by BP IgG1. These results indicate that BP IgG2 and IgG4 have a capability to inhibit the increase of [Ca2+]i induced by BP IgG1. |
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