兔移植性子宫内膜肿瘤模型的建立及其生物学特性观察

目的：建立子宫内膜VX2肿瘤模型并观察其生物学特性。方法：采用子宫内膜分离组织包埋法在44只兔的子宫内膜制备VX2肿瘤模型，24只在1～6周处死，观察其生长及转移特性，20只用于观察模型自然生存期。结果：肿瘤原位移植成功率为100％；1周内肿瘤局限于子宫内膜，1～2周侵犯肌层并发生淋巴转移，2～3周浸润子宫浆膜并向同侧宫角蔓延，3～4周发生宫颈转移，4～5周发生肺转移；荷瘤兔的自然生存时间为50．6±8．1d，其转移灶与原发灶组织学类型一致，均为鳞癌。结论：此模型移植成功率高，生物学特性稳定，其转移模式与人子宫内膜肿瘤转移方式类似，为子宫内膜肿瘤的实验研究提供了较理想的大动物模型。

Establishment of transplanted endometrial neoplasm model in rabbit and its biological features

objective: To establish a endometrial neoplasm model in rabbits and observe its biological features. Methods:VX2 tumor grafts were established by orthotopic embeding en-dometrium of rabbits and the biological features were observed by macroscopical and histological examination. Results: The orthotopic tumor growth rate was 100%. Tumors were restricted in the endometrium within one week after local transplantation: infiltraed the myometrium and the serosa, metastasized to lymphnodes in 1 to 3 weeks. Cervical and lung metastases appeared in 3- 4 and 4 - 5 weeks respectively. The average survival time was 50. 6 ±8. 1days. The histolog-ical types of the primary and metastatic foci were similar. Both belonged to squamous cell carci-noma. Conclusions: This model has a high success rate and a steady biological feature. The pat-tern of metastases is similar to that of the human endometrial neoplasm, so it serves as a good model for the study of endometrial neoplasm.