Group-I intron family in the nuclear ribosomal RNA small subunit genes ofCenococcum geophilum isolates |
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| Authors: | Mari L. Shinohara Katherine F. LoBuglio Scott O. Rogers |
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| Affiliation: | (1) College of Environmental Science and Forestry, State University of New York, 1 Forestry Drive, 13210-2788 Syracuse, NY, USA;(2) Present address: Department of Biochemistry, Dartmouth Medical School, 03755-3844 Hanover, NH, USA;(3) Present address: Department of Plant Biology, University of California, 94720 Berkeley, CA, USA |
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| Abstract: | A family of optional group-I introns was found near the 3 end of the nuclear small subunit rRNA genes in 61 out of 70 isolates of the deuteromycete mycorrhizal fungusCenococcum geophilum. DNA sequence polymorphisms among the introns (termedCgSSU introns) from ten of the isolates were studied. The sequences, ranging in size from 488 to 514 nucleotides, were from 93.2% to 99.6% similar to each other. Mutations were less common in predicted base-paired regions (33% of all mutations) than in free-standing regions (67%). The introns were self-spliced in vitro and were closest to subgroup ICI according to sequence and predicted secondary structure. Group-I intron pairing regions P1 through P10, including core regions P, Q, R and S, were present in all tenCgSSU introns studied. No lengthy open reading frames were found in any of the introns, indicating that the introns do not encode a protein, and therefore may not be mobile. It is likely that a single intron entered a progenote ofC. geophilum and changed as the species evolved. |
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| Keywords: | Self-splicing Group-I intron Ribosomal RNA Cenococcum geophilum |
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