诊断超声对不同发育阶段大鼠睾丸组织凋亡的研究

目的探讨诊断超声对不同发育阶段大鼠睾丸组织生精细胞凋亡的影响。方法３６只ＳＤ雄性大鼠随机分为６组。应用ＨＰ８５００彩超仪对大鼠睾丸进行３０ｍｉｎ持续照射，２４ｈ后取睾丸进行凋亡检测。结果不同年龄组生精细胞均有不同程度的细胞凋亡，以出生后３０ｄ凋记细胞数最多，随着龄增长凋亡细胞数有减少趋势，超声照射３０ｍｉｎ后各实验组凋亡细胞数均有显著增加。结论不同发育阶段大鼠睾丸生精细胞凋亡率不同，超声照射３０ｍ

Study of Apoptosis in Different Age Rat Testes af ter Diagnostic Ultrasound Radiation

Objective To investigate the germ cell apoptosis in different age rat testes after diagnostic ultrasound. Methods 36 male Sprague|Danles rats were randomly separated into six groups of six according to different ages. Rat testes were radiated by HP 8500 color Doppler sonography with 7.5MHz linear phased array transducer for 30 minutes. Their testes were removed at 24 hour after radiation. Apoptosis was detected with TUNEL (in situterminal deoxynucleotityl transferase|mediated dUTP nick end labeling). Results The incidence of testicular germ cell apoptosis in all groups was detected and peaked at post|birth 30 days;after radiation apoptotic incidence in all experimental groups was significantly higher than control groups ( P <0 001). Conclusion Germ cell apoptotic incidence varied in different age rat; radiation continuously 30 minutes by diagnostic ultrasound may result in germ cell apoptosis increasing in the different age rat testes. [