低频振动对人成骨细胞生物学特性影响的研究

目的:研究低频振动对人成骨细胞增殖分化及基质分泌的影响。方法:取成人的髂骨松质骨,获得人成骨细胞。并在培养48h后,施加0.1、0.2、0.5、2和5Hz的低频微振动,通过流式细胞仪检测成骨细胞增殖状况:化学比色法和放射免疫法检测碱性磷酸酶(ALP)活性和骨钙素含量。结果:加载0.2和0.5Hz可使成骨细胞的ALP活性明显增高(P<0.01);0.1和2Hz振动后其ALP活性与对照组无明显差异;5Hz振动则明显降低ALP活性(P<0.01)。加载0.2和0.5Hz振动的成骨细胞,S期细胞从10.4％增加至12.45％和16.12％,增殖指数从20.14％增加到26.21％和28.75％。0.1和2Hz低频振动则显示出对细胞增殖指数无明显影响(P>0.05)。而5Hz使细胞增殖指数明显降低至13.22％(P<0.05)。同时,加载0.2和0.5Hz振动可明显增加成骨细胞骨钙素分泌量至1.87和2.47ng/ml(P<0.05),而加载0.1Hz对骨钙素分泌量无明显影响(P>0.05),加载2和5Hz相应降低了骨钙素分泌量(P<0.05)。结论:0.2～0.5Hz的低频振动能促进成骨细胞增殖分化和成骨活性物质分泌,对低频振动应用于骨折治疗有指导作用。

Effects of Low Frequency Vibration on Human Osteoblasts

Objective:To study the effect of low frequency vibration on cultured human osteoblasts in proliferation and differentiation. Methods: Osteoblasts isolated from the iliac trabecular bone specimens were treated with collagenase-trypsin, were then purified and cultured,and subsequently,osteoblasts were dealt with under low frequency vibration(0.1,0.2,0.5,2 and 5Hz)for 5d. The flow cytomety were used to observe the effect on the proliferation of osteoblasts. The biochemical indexes - alkaline phosphatase(ALP) and osteocalcin were assayed by spectrohotometric methods and radioimmunoassay respectively. Results :0.2 and 0.5Hz vibration could markedly augment osteoblast ALP activity(P<0.05) ;0.1 and 2Hz vibration had no obvious effects on osteoblast ALP activity(P<0. 05);5Hz distinctly reduced ALP activity (P<0.05).0.2 and 0. 5Hz vibration could significantly increase cellnumbers of S phase from 10.4% to 12.45% and 16.12% , PI value from 20.14% to 26.21% and 28.75% respectively; 0. 1 and 2Hz vibration had no obvious effects on osteoblast proliferation index; 5Hz obviously decreased PI value to 13.22%(P<0.01). 0.2 and 0.5Hz vibration could increase OCN release to 1.87 and 2.47ng/ml while, 2Hz and 5Hz all decrease it markedly(P < 0.05). Conclusion: 0.2 - 0. 5Hz vibration could accelerate the osteoblast proliferation and differentiation, as well as ALP activity and OCN release.