| 几种生长因子在人表皮细胞无血清培养中的作用 |
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| 引用本文： | 刘德伍,陈国安,等.几种生长因子在人表皮细胞无血清培养中的作用[J].江西医学院学报,1998,38(2):41-44. |
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| 作者姓名： | 刘德伍 陈国安 |
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| 作者单位： | 江西医学院第一附属医院烧伤科南昌３３０００６）陈国安戴育成（江西省医学科学研究所 |
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| 摘 要： | 采用无血清培养系统，以３Ｈ－ＴｄＲ掺入法测定表皮生长因子（ＥＧＦ）、牛垂体提取物（ＢＰＥ）和乙醇胺（Ｅｔｈａ）对人表皮细胞ＤＮＡ合成的影响。结果显示，ＥＧＦ和ＢＰＥ在本实验所建立的无血清培养基中对培养表皮细胞ＤＮＡ合成有明显的促进作用（Ｐ＜０．０１）；Ｅｔｈａ对培养表皮细胞ＤＮＡ合成也有促进作用（Ｐ＜０．０５），ＥＧＦ和Ｅｔｈａ在本培养体系中具有协同作用（Ｐ＜０．０５）。讨论了生长因子的作用机理及在烧伤创面的应用前景
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| 关 键 词： | 生长物质／生理学 培养基.无血清 表皮 人 |
The Roles of Several of Growth Factors in Serum free Culture of Human Epidermal Cells in Vitro |
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| Liu Dewu,Cao Yong,Li Guohui,Su Ziyi.The Roles of Several of Growth Factors in Serum free Culture of Human Epidermal Cells in Vitro[J].Acta Academiae Medicinae Jiangxi,1998,38(2):41-44. |
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| Authors: | Liu Dewu Cao Yong Li Guohui Su Ziyi |
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| Abstract: | This study was established in serum free condition.The effects of epidermal growth factor (EGF),bovine pituitary extract (BPE), and ethanolamine (Etha) on the DNA synthesis of human epidermal cells were determined by thymidine incorporation.EGF and BPE were shown to promote the DNA synthesis of cultured epidermal cells significantly ( P <0 01).Etha exerted a positive influence on it ,too (P <0.05).A mixture of EGF and Etha yielded better growth than either alone ( P <0.05).The possible mechanism and applied perspective of the growth factors used for burn were discussed. |
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| Keywords: | growth substances/physiology culture media serum free epidermis men |
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