微卫星锚定PCR分析19个湖北钉螺种群之间的遗传变异关系

目的探讨湖北钉螺种群间的遗传变异关系。方法采用微卫星锚定PCR分子标记技术对来自中国大陆8个省的19个种群钉螺基因组DNA进行扩增，分析钉螺各种群间的遗传变异并对钉螺种群进行聚类分析。结果19个钉螺种群间的遗传距离D在0～0．73之间，平均遗传距离D为0．22±0．013。19个钉螺种群被聚成4类，一类包括分布在长江中下游流域（湖南、湖北、江西、安徽、江苏）的16个钉螺种群；广西宜州、福建福清和云南大理的钉螺种群分别单独成一类。结论目前分布在中国大陆的湖北钉螺可能分为4个亚种，即指名亚种、滇川亚种、福建亚种、广西亚种。

Genetic diversity in 19 Chinese populations of Oncomelania hupensis(Gastropoda:Rissooidea)detected by simple sequence repeat-anchored polymerase chain reaction amplification

OBJECTIVE: To explore the genetic diversity among populations of Oncomelania hupensis. METHODS: Simple sequence repeat-anchored PCR method was used to amplify the genomic DNA of nineteen snail populations from 8 provinces, and the genetic diversities among these snail populations were analyzed. RESULTS: The genetic distance D among nineteen snail populations ranged from 0 to 0.73 with average genetic distance as D 0.22 +/- 0.013. All the 19 snail populations were clustered into four groups: one group included 16 snail populations distributed throughout the Yangtze River drainage system below the Three Gorges of the river (Hunan, Hubei, Jiangxi, Anhui, and Jiangsu provinces); Snail populations from Yizhou city, Guangxi province, Fuqing city from Fujian province, and Dali city from Yunnan province belonged to an independent group respectively. CONCLUSION: The distribution of Oncomelania hupensis in mainland China could be divided into four subspecies, i.e. Oncomelania hupensis hupensis (O. h. hupensis ), O. h. robertsoni, O. h. tangi , and O. h. guangxiensis.