针刺对胶原诱导性关节炎大鼠胃肠动力的影响

目的：利用制备的胶原诱导性关节炎(CIA)大鼠模型，探讨针刺足三里对CIA大鼠胃肠动力的影响。方法：将60只12周龄雄性大鼠随机分为4组：正常组、模型组、电针组、手针组，每组15只，建立牛Ⅱ型胶原与不完全弗氏佐剂诱导CIA大鼠模型。电针组和手针组于造模完成后第1天取足三里穴治疗，15 min/次，1次/d,连续治疗14 d。在3 d, 7 d, 14 d时，采用跖围法测大鼠跖围长度，并在治疗14 d后，用苏木精-伊红(HE)染色观察膝关节的病理学变化，计算大鼠的胃排空率和肠推进率，结肠蠕动波测定方法检测大鼠的肠蠕动波次数，通过蛋白质免疫印迹法检测胃黏膜中促肾上腺皮质激素释放因子(CRF)和血管活性肠肽(VIP)的表达。结果：与正常组比较，模型组大鼠跖关节肿胀明显(P<0.01),软骨组织破坏严重并有炎症细胞浸润，胃排空率和肠推进率均降低(P<0.01),胃黏膜中CRF和VIP表达明显增多(P<0.01)。与模型组比较，针刺后大鼠跖关节肿胀程度明显降低(P<0.05),软骨破坏程度减轻和炎症细胞浸润明显减少，胃排空率和肠推进率提升(P<0.05),肠蠕动波次...

Effect of Acupuncture at Zusanli(ST 36) on Gastrointestinal Motility in Rats with Collagen-induced Arthritis

To investigate the effect of acupuncture at Zusanli(ST 36) on gastrointestinal motility in the rats with collagen-induced arthritis(CIA).Methods:Sixty healthy Sprague-Dawley(SD) rats aged 3 months were randomly assigned into four groups:normal group,model group,electroacupuncture group,and hand acupuncture group,with 15 rats in each group.Electroacupuncture and hand acupuncture were respectively carried out at Zusanli(ST 36) for 15 min once a day for 14 days since the first day after modeling.On days 3,7,and 14,plantar circumference of each rat was measured.At the end of treatment,the pathological changes of knee joints were observed by hematoxylin-eosin(HE) staining.The gastric emptying rate and intestinal propulsion rate were calculated,and the frequency of peristaltic waves in intestine was detected.The expression of corticotropin-releasing factor(CRF) and vasoactive intestinal peptide(VIP) in gastric mucosa was measured.Results:Compared with the normal group,the modeling caused the swelling of plantar joint(P<0.01),severe damage of cartilage tissue,and infiltration of inflammatory cells,decreased the gastric emptying rate and intestinal propulsion rate(P<0.01),and up-regulated the expression levels of CRF and VIP in gastric mucosa(P<0.01).Compared with the model group,the acupuncture at Zusanli(ST 36) alleviated the plantar joint swelling(P<0.05),cartilage damage,and inflammatory cell infiltration,increased the gastric emptying rate and intestinal propulsion rate(P<0.05),accelerated intestinal peristalsis,and down-regulated the expression of CRF and VIP in gastric mucosa(P<0.05).In particular,hand acupuncture demonstrated better performance.Conclusion:Acupuncture at Zusanli(ST 36) can regulate intestinal motility disorder in CIA rats,and hand acupuncture has better therapeutic effect than electroacupuncture.