莱菔硫烷对汞致大鼠肾急性损伤的保护作用

目的 探讨莱菔硫烷(sulforaphane,SFN)对莱菔硫烷(sulforaphane,SFN)所致大鼠急性肾损伤的保护作用。 方法 清洁级Wistar大鼠30只按体重随机分5组,每组6只,分别为对照组,低、中、高剂量染汞组, SFN干预组。对照组及各染汞组皮下注射生理盐水,SFN干预组皮下注射2 mg/kg SFN;2 h后,对照组腹腔注射生理盐水,其他四组腹腔注射2.2、4.4、8.8、8.8 μmol/kg HgCl₂,连续3 d,染毒容量均为5 ml/kg。于最后一次染毒结束后,测定尿液中Hg及尿蛋白含量,碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)和β-N-乙酰氨基葡萄糖苷酶(NAG)活力;腹主动脉采血测定血清尿素氮(BUN)含量;测定肾皮质中Hg含量和谷胱甘肽(GSH)、丙二醛(MDA)水平及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力。分析各组各项指标差异。 结果 与对照组比较,各剂量染汞组大鼠尿Hg、肾皮质Hg含量,尿蛋白和血清BUN 含量,尿NAG、LDH 和ALP 活力,肾皮质中GSH 和MDA含量显著升高(P<0.05或P<0.01);肾皮质SOD 和GSH-Px 活力显著降低(P<0.05或P<0.01)。与8.8 μmol/kg HgCl₂组比较,SFN 干预组尿蛋白和血清BUN 含量、尿NAG、LDH 和ALP活力、肾皮质GSH 、MDA含量均显著下降(P<0.05或P<0.01);肾皮质SOD 和GSH-Px 活力显著升高(P<0.01),而尿汞、肾皮质汞含量间差异无统计学意义(P>0.05)。 结论 莱菔硫烷对汞所致大鼠急性肾毒性有一定的保护作用。

Protective effect of sulforaphane on mercury-induced acute renal injury in rats

Objective To explore the protective effect of sulforaphane (SFN) on acute renal injury induced by mercury (Hg) in rats. Methods Thirty clean Wistar rats were randomly assigned to 5 groups based on their body weight, including the control group, low-, middle- and high-dose mercury exposed groups and SFN pre-treatment group, with 6 rats for each group. The rats in the control group and the three mercury exposed groups were subcutaneously injected with saline, while those in the SFN pre-treatment group received a subcutaneous injection of 2 mg/ml SFN. 2 hours later, the rats in the control group were intraperitoneally injected with saline, while those in the other four groups were intraperitoneally injected with 2.2 μmol/kg, 4.4 μmol/kg, 8.8 μmol/kg and 8.8 μmol/kg HgCl₂ respectively. The rats were treated as above for 3 successive days. The exposure volume was 5 ml/kg. After the last administration, the contents of urinary mercury and protein as well as the activities of urinary alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and β-N-acetyl-D-glucosaminidase (NAG) were determined. Blood was collected from the abdominal aorta for determination of blood urea nitrogen (BUN). Meanwhile, the levels of mercury, glutathione(GSH) and malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the renal cortex were detected. The differences of the indicators among the groups were analyzed. Results Compared with the rats in the control group, the contents of urinary Hg and protein, of the renal cortex Hg, urinary and serum BUN, as well as the activities of urinary NAG, LDH and ALP and the contents of GSH and MDA in the renal cortex of the rats in the mercury exposed groups increased significantly (P<0.05 or P<0.01), while the activities of SOD and GSH-Px in the renal cortex markedly decreased(P<0.05 or P<0.01). Compared with the rats exposed to 8.8 μmol/kg HgCl₂, the contents of urinary protein and serum BUN, the activities of urinary NAG, LDH and ALP and the contents of GSH and MDA in the renal cortex of the rats in the SFN pre-treatment group were significantly decreased (P<0.05 or P<0.01), while the activities of SOD and GSH-Px in the renal cortex were markedly increased (P<0.01). But no statistically significant differences were found in the contents of Hg in the urine and renal cortex (P>0.05). Conclusion SFN has a protective effect on mercury-induced acute renal toxicity in rats.