线性探针技术快速诊断本溪地区耐多药肺结核效果评价

目的 评价线性探针技术(LPA)在本溪地区快速检测结核分枝杆菌利福平和异烟肼耐药性的特异性和敏感性。 方法 采用传统罗氏培养法培养2013年3月-2014年6月期间全市县区所有登记的414例涂阳肺结核患者痰标本,获得菌株经比例法药物敏感性试验和线性探针技术检测利福平和异烟肼耐药性,并以比例法药物敏感性试验为金标准分析线性探针方法的特异性和敏感性。 结果 经培养获得394株结核分枝杆菌菌株,与比例法药物敏感性试验相比较,线性探针技术检测利福平和异烟肼的特异性分别为97.9%和96.6%,敏感性分别为91.1%和81.3%;对耐多药检测的特异性和敏感性分别为98.8%和89.9%。 结论 线性探针技术是一快速、敏感、特异的诊断结核分枝杆菌利福平、异烟肼耐药和耐多药(MDR)的有效方法,可在本溪地区推广应用。

Evaluation on the line probe assay for rapid diagnosis of multidrug-resistant tuberculosis in Benxi region

Objective To evaluate the specificity and sensitivity of the line probe assay in rapid detection of resistance to rifampin (RFP) and isoniazid (INH) in Mycobacterium tuberculosis in Benxi region. Methods Four hundred and fourteen sputum specimens from all registered smear-positive tuberculosis patients in the whole city were cultured by traditional Lwenstein-Jensen culture from March 2013 to June 2014. The positive cultures were examined by traditional drug sensitivity test, and the resistance to RFP and INH was determined by the line probe assay. The traditional drug sensitivity test as the golden standard was used to analyze the specificity and sensitivity of the line probe assay. Results Three hundred and ninety-four Mycobacterium tuberculosis strains were obtained. Compared with the traditional drug sensitivity test, the specificity and sensitivity of detecting resistance to RFP and INH and multi-drug resistance by the linear probe assay were 97.9% and 91.1%, 96.6% and 81.3%, 98.8% and 89.9% respectively. Conclusions The line probe assay is a rapid, sensitive, specific and effective method for the diagnoses of resistance to RFP and INH and multi-drug resistance in Mycobacterium tuberculosis. It can be popularized and applied in Benxi region.