| HPLC法测定辽东楤木根皮中木皂苷C的含量 |
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| 引用本文： | 宋少江,李丽,马志强,徐绥绪. HPLC法测定辽东楤木根皮中木皂苷C的含量[J]. 沈阳药科大学学报, 2006, 0(3) |
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| 作者姓名： | 宋少江 李丽 马志强 徐绥绪 |
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| 作者单位： | 沈阳药科大学中药学院 辽宁沈阳110016 |
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| 基金项目： | 辽宁省博士启动基金项目 |
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| 摘 要： | 目的建立HPLC法测定辽东木根皮中木皂苷C含量的方法。方法固定相为DiamonsinC18柱(250 mm×4.6 mm,5μm),流动相为甲醇水磷酸(体积比为67.0∶33.0∶0.2),流速为0.8 mL.min-1,检测波长为206 nm,柱温为30℃。结果木皂苷C在20~40μg内呈良好的线性关系(r=0.999 9),平均回收率为99.7%,RSD为3.2%(n=6)。结论可用于木皂苷C的定量分析。
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| 关 键 词： | 辽东楤木 木皂苷C 高效液相色谱 |
Determination of araloside C in root barks of Aralia elata(Miq.)Seem.by HPLC |
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| SONG Shao-jiang,LI Li,MA Zhi-qiang,XU Sui-xu. Determination of araloside C in root barks of Aralia elata(Miq.)Seem.by HPLC[J]. Journal of Shenyang Pharmaceutical University, 2006, 0(3) |
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| Authors: | SONG Shao-jiang LI Li MA Zhi-qiang XU Sui-xu |
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| Abstract: | Objective To develop the analysis method for the determination of the content of araloside C in the root bark of Aralia elata(Miq.)Seem.by HPLC.Methods The chromatographic condition was Diamonsin C_(18) column(250 mm×4.6 mm,5 μm).A mixture of methanol-water-phosphoric acid(V∶V∶V=(63.0∶)37.0∶0.2)was used as the mobile phase with a flow rate of 0.8 mL/min and the detection wavelength was set at 206 nm at 30 ℃.Results The araloside C curve showed good linear correlation within the concentration range of(20～)40 μg(r=0.999 9).The average recovery and RSD was 99.7% and 3.2%((n=6)) respectively.Conclusions The method is convenient,reliable and with good reappearance. |
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| Keywords: | Aralia elata(Miq.) Seem. araloside C HPLC |
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