乌司他丁对大鼠百草枯中毒急性肺损伤的治疗作用

目的评估乌司他丁对大鼠百草枯(PQ)中毒急性肺损伤的影响。方法 35只雄性SD大鼠,按随机数字表法分成正常对照组(A组)5只、PQ中毒模型组(B组)15只、乌司他丁组(C组)15只。B组与C组禁食1 h后经口一次灌胃PQ 75 mg/kg制成百草枯中毒急性肺损伤模型,A组经口一次性注入等量生理盐水;C组腹腔注射乌司他丁10万单位/d,共3 d,A组与B组给予0.2 ml/d生理盐水腹腔注射。A组于造模后24 h,B、C两组大鼠于造模后24、48、72 h分别取5只大鼠肺组织与血清。肺组织行HE染色,血清测定肿瘤坏死因子-α(TNF-α)、转化生长因子β1(TGF-β1)。结果 B组血清TNF-α在第24小时即出现显著统计学差异,且持续存在;血清TGF-β1造模后24 h即升高,在第48小时与A组比较有差异,在72 h则出现显著统计学差异(P<0.01)。C组与B组比较,血清TNF-α升高缓慢,在第24小时即有统计学差异(P<0.05),持续至第72小时;而两组大鼠的TGF-β1比较却无统计学差异(P>0.05)。结论乌司他丁可以减轻百草枯中毒急性肺损伤,但对TGF-β1的影响有限,可能通过其他途径减缓中毒后的肺纤维化过程。

Experimental study on therapeutic effect of Ulinastatin on acute lung injury of rats associated with Paraquat poisoning

Objective To investigate the preventive effects of Ulinastatin(UTI) on acute lung injury(ALI) of rats associated with Paraquat(PQ) poisoning.Methods A total of 35 SD rats were divided into control group(Group A,n = 5),PQ group(Group B,n = 15) and UTI group(Group C,n = 15) according to random number table.Group B and Group C were induced by PQ feeding(75 mg/kg) after one hour’s fasting so as that ALI models would be made.Group A was fed with an equal amount of normal saline.Group C received UTI(100,000 U / d,once daily) via intraperitoneal injection for three days,and Group A and Group B received normal saline(0.2 mL / d).Lung tissue specimens and sera were taken from Group A(at 24 hours) and from Group B and Group C(at 24-,48-and 72 hours),respectively.The lung tissue specimens were treated with HE staining.The contents of tumor necrosis factor-α(TNF-α) and transforming growth factor-β1(TGF-β1) in the sera were measured.Results The TNF-α of Group B significantly increased at 24 hours and at the later hours,and the TGF-β1 began to increased at 24 hours,significantly increased at 48 hours and more significantly increased at 72 hours when compared with that of Group A(P < 0.01).Compared with that of Group B,the TNF-α of Group C increased slowly at 24 hours(lasting to 72 hours)(P < 0.05),and the TGF-β1 was of no significant difference(P > 0.05).Conclusion UTI can reduce ALI caused by PQ poisoning.It may slow pulmonary fibrosis after poisoning by other means,in consideration of its limited influence on TGF-β1.