| HPLC法测定金银冠心口服液中绿原酸含量的研究 |
| |
| 引用本文： | 崔斌,朱庆松,李明姬,朱寅圣. HPLC法测定金银冠心口服液中绿原酸含量的研究[J]. 实用中医内科杂志, 2009, 23(10): 28-29. DOI: 10.3969/j.issn.1671-7813.2009.10.14 |
| |
| 作者姓名： | 崔斌 朱庆松 李明姬 朱寅圣 |
| |
| 作者单位： | 延边大学医学部,吉林延吉,133000 |
| |
| 基金项目： | 金银冠心口服液是延边大学与延边州合作开发项目 |
| |
| 摘 要： | [目的]采用高效液相色谱法测定金银冠心液中绿原酸的含量。[方法]用YMC-Pack OSD-A C_(18)色谱柱(250mm ×4.6mm 5μm),甲醇-水-冰醋酸(22:78:1)为流动相,流速0.8mL·min~(-1),检测波长327nm。[结果]绿原酸在0.575-2.3μg范围内呈良好线性关系(r=0.9999),平均回收率为100.01%(RSD=1.68%,n=5)。[结论]本方法简便、快速、可靠,可用于金银冠心液的质量控制。
|
| 关 键 词： | 金银冠心液 HPLC 绿原酸 |
Determination of Chlorogenic acid in JinYinGuanXin solution by HPLC |
| |
| CUI Bin,ZHU Qing-song,LI Ming-ji,ZHU Yin-sheng. Determination of Chlorogenic acid in JinYinGuanXin solution by HPLC[J]. , 2009, 23(10): 28-29. DOI: 10.3969/j.issn.1671-7813.2009.10.14 |
| |
| Authors: | CUI Bin ZHU Qing-song LI Ming-ji ZHU Yin-sheng |
| |
| Affiliation: | CUI Bin ZHU Qing-song LI Ming-ji ZHU Yin-sheng |
| |
| Abstract: | [ Objective]To determine Chlorogenic acid content in JinYinGuanXin solution by HPLC. [Methods ] The determination was carried out with YMC -Pack OSD -A C18 column (250mm ×4. 6mm 5μn) with methanol: water; acetic acid(22 :78 : l)as the mobile phase; the flow rate was 0.8ml ·min-1 ,the detective wavelength was set at 327nm. [ Results ] The Chlorogenic acid curve was linear over the range of 0. 575 - 2. 3 μg( r =0. 9999 ) , the average recovery was 100. 01 % ( RSD = 1. 68% ,n =5).[ Conclusions ] The method was simple, rapid, accurate and reliable , It can be used for quality control of JinYinGuanXin solution. |
| |
| Keywords: | HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
