Elevated cell-associated levels of interleukin 1β and interleukin 6 in inflamed mucosa of inflammatory bowel disease |
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Authors: | E. GRÖ TTRUP-WOLFERS,,J. MOELLER,U. KARBACH,S. MÜ LLER-LISSNER,& S. ENDRES |
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Affiliation: | Virchow-Klinikum, Humboldt-Universität Berlin, Berlin;,;Medizinische Klinik, Klinikum Innenstadt, Ludwig-Maximilians-Universität, Munich;,;Städtisches Krankenhaus Berlin-Weissensee, Berlin, Germany |
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Abstract: | The aim of this study was to investigate the involvement of the monocyte-derived cytokines interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumour necrosis factor-α (TNF-α) in idiopathic inflammatory bowel disease. Endoscopic biopsies of normal and inflamed intestinal mucosa were obtained from patients with ulcerative colitis ( n = 11) and with Crohn's disease ( n = 10). Intestinal mucosal cells were isolated by collagenase digestion. Cell viability, morphology and CD14 expression were determined. To measure cell-associated cytokine levels, cells were lysed and analysed for IL-1β and TNF-α in specific radioimmunoassays and for IL-6 using a biological assay. Compared with mucosal cells from control patients without inflammatory bowel disease the inflamed intestine in ulcerative colitis and Crohn's disease displayed markedly enhanced levels of IL-1β (median 245 pg 10−6 cells, range 30–1275) and IL-6 (median 22 U 10−6 cells, range 1–298). Non-inflamed mucosa in patients with ulcerative colitis and Crohn's disease did not shown elevated levels of IL-1β (median 50 pg 10−6 cells, range 33–90) or IL-6 (mean below detection limit of assay, i.e. 1 U 10−6 cells). In contrast, no clear cut difference between inflamed and non-inflamed mucosa could be detected for TNF-α. High tissue levels of IL-6 were associated with a high endoscopic grade of local inflammation. These results suggest that the monocyte-derived cytokines IL-1β and IL-6 are mediators of inflammation in inflammatory bowel disease. |
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Keywords: | Inflammatory bowel disease interleukin 1 interleukin 6 tumour necrosis factor |
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