不同睡眠剥夺时间对大鼠海马和前额皮层Ng、PKC、CaMKⅡ mRNA表达的影响

目的：探讨不同睡眠剥夺时间对大鼠海马和前额皮层神经颗粒素（Ng)、蛋白激酶C (PKC)、 Ca2+ -CaM依赖性蛋白激酶Ⅱ（CaMKⅡ）mRNA表达的影响。方法： 雄性Wistar大鼠随机分为3组，即24 h实验组、48 h实验组和72 h实验组，每组再分为睡眠剥夺组（SD组）和对照组（C组）。利用睡眠剥夺箱建立大鼠SD模型，Trizol一步法提取海马和前额皮层总RNA，用SYBRA　green Ⅰ荧光定量RT-PCR测定Ng、PKC和CaMKⅡ mRNA水平。结果： (1)大鼠海马Ng mRNA水平在睡眠剥夺24 h、48 h和72 h后，较对照组均显著降低（P＜0.05），PKC和CaMKⅡ mRNA水平在睡眠剥夺48 h和72 h后显著降低（P＜0.05）； (2)在睡眠剥夺24 h和48 h后，大鼠前额皮层Ng、PKC和 CaMKⅡ mRNA水平较对照组有降低趋势，在睡眠剥夺72 h后均显著降低（P＜0.05）。结论： 睡眠剥夺可以使大鼠海马和前额皮层Ng、PKC和CaMKⅡ mRNA表达水平降低，且随着睡眠剥夺时间的延长，降低更为明显，这可能是睡眠剥夺损害认知功能的原因之一。

Effects of different duration of sleep deprivation on neurogranin, PKC and CaMK II mRNA expression in hippocampus and forebrain of rats

AIM: To study the effects of different duration of sleep deprivation (SD) on neurogranin (Ng), protein kinase C (PKC) and Ca2+-calmodulin dependent protein kinase II (CaMK II) mRNA expressions in hippocampus and forebrain of rats. METHODS: Male Wistar rats were divided into three groups: 24 h, 48 h and 72 h experimental groups. Each group was divided into sleep deprivation group (SD group) and control group (C group). SD model was established by sleep deprivation box. One step methods by Trizol was used to extract hippocampus and forebrain neuronal total RNA. The changes of Ng, PKC and CaMK II mRNA expressions were detected by SYBRA green I RT-PCR. RESULTS: (1) The expression of Ng mRNA in hippocampus of SD group was significantly lower than that in C group after 24 h, 48 h and 72 h SD (P<0.05). The expressions of PKC and CaMK II mRNA in SD group were significantly lower than those in C group after 48 h and 72 h SD (P<0.05). (2) The expressions of Ng, PKC and CaMKⅡ mRNA in forebrain of SD group were lower than those in C group after 24 h and 48 h SD，and significantly lower than those in C group only after 72 h SD (P<0.05). CONCLUSION: The expressions of Ng, PKC and CaMKⅡ mRNA are down regulated by SD, which might be relevant to the impairment of cognitive function in rats.