| RT-PCR分析工频磁场暴露后MCF-7细胞p53 mRNA的表达 |
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| 引用本文： | 丁桂荣,郭国祯,郭鹞,宫越顺二. RT-PCR分析工频磁场暴露后MCF-7细胞p53 mRNA的表达[J]. 医学争鸣, 2002, 23(11): 1016-1018 |
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| 作者姓名： | 丁桂荣 郭国祯 郭鹞 宫越顺二 |
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| 作者单位： | 第四军医大学预防医学系放射医学教研室,陕西,西安,710033;日本京都大学医学部放射遗传学教研室 |
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| 摘 要： | 目的：观察工频磁场暴露对MCF－7细胞p53mRNA表达的影响。方法：将人乳腺癌MCF－7细胞暴露或假暴露于60Hz,5mT工频场中4，8和24h，采用RT－CR方法定量分析照射后不同时间MCF－7细胞p53mRNA的表达水平。结果：工频磁场暴露4，8和24h，MCF－7细胞p53mRNA的表达水平保持不变，结论：工频磁场暴露对MCF－7细胞p53mRNA的表达没有影响。
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| 关 键 词： | 工频磁场 MCF-7细胞 RT-PCR p53 |
| 文章编号： | 1000-2790(2002)11-1016-03 |
| 修稿时间： | 2001-12-06 |
Analysis of p53 mRNA expression in MCF-7 cells exposed to power-frequency magnetic fields |
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| DING Gui Rong ,GUO Guo Zhen ,GUO Yao ,Miyakoshi Junji. Analysis of p53 mRNA expression in MCF-7 cells exposed to power-frequency magnetic fields[J]. Negative, 2002, 23(11): 1016-1018 |
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| Authors: | DING Gui Rong GUO Guo Zhen GUO Yao Miyakoshi Junji |
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| Affiliation: | DING Gui Rong 1,GUO Guo Zhen 1,GUO Yao 1,Miyakoshi Junji 2 1Department of Radiation Medicine,Faculty of Preventive Medicine,Fourth Military Medical University,Xi'an 710033,China,2Department of Radiation Genetics,Graduate School |
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| Abstract: | AIM To observe effects of power frequency magnetic fields on p53 mRNA expression in MCF 7 cells. METHODS Human breast cancer MCF 7 cells were exposed or sham exposed to 60 Hz, 5 mT magnetic field for 4, 8 and 24 h, and then p53 mRNA expression was detected by using RT PCR method. RESULTS p53 mRNA expression in MCF 7 cells was not altered after exposure to magnetic fields. CONCLUSION Exposure to power frequency magnetic fields has no effects on p53 mRNA expression. |
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| Keywords: | RT-PCR p53 |
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