正常皮肤,增生性瘢痕和瘢痕疙瘩成纤维细胞培养,生物 …

目的 探讨正常皮肤、增生性瘢痕和瘢痕疙瘩成纤维细胞的体外培养、生物学特征及超微结构、阐明其应用价值。方法 采用成纤维细胞体外培养技术，从正常皮肤成纤维细胞与增生性瘢痕，瘢痕疙瘩成纤维细胞在细胞增殖，细胞形态，细胞遗传学特征及细胞超微结构方面进行比较研究。结果 体外培养的正常皮肤成纤维细胞与增生性瘢痕、瘢痕疙瘩成纤维生长率和形态学相同，细胞遗传学特征和超微结构相似。结论 据此结果和其他学者的观点，认

Comparative study on biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloidin in vitro culture]

OBJECTIVE: This study was to investigate the in vitro cultivation, biological characteristics and ultrastructure of the fibroblasts derived from normal skin, hypertrophic scar and keloid, to elucidate the value of their application. METHOD: A comparative study was performed on cell proliferation, cell morphology, cytogenetic feature and cell ultrastructure of fibroblasts from normal skin, hypertrophic scar and keloid by means of the technique of in vitro culture. RESULT: The results indicated that the fibroblasts isolated from normal skin, hypertrophic scar and keloid exhibited similar morphology and growth rates. The cytogenetic feature and cell ultrastructure were also alike. CONCLUSION: According to our study and that of others, we conclude that it is entirely feasible to establish an in vitro culture model of fibroblasts from normal skin for investigation, prevention and treatment of scars.