重组脂联素在肥胖和2型糖尿病小鼠体内分布研究

研究脂联素球形结构域重组蛋白(gAcrp)在饮食诱导肥胖(DIO)小鼠、2型糖尿病(T2DM)dx鼠和正常小鼠体内组织分布.用125Ⅰ-gAcrp示踪方法测定静脉注射后0.5h和2h时标记物在各组小鼠体内组织分布.结果表明,与0.5h相比,2h时125Ⅰ-gAcrp在T2DM小鼠肝脏和DIO小鼠颌下腺中的分布明显提高,在DIO小鼠肝脏中 明显减少,并在DIO小鼠睾丸、胃、肠和T2DM小鼠子宫中的分布有所增加.除文献报道的肝脏、骨骼肌外,颌下腺、睾丸、子宫也可能是脂联素在肥胖和T2DM个体中发挥作用的重要器官.

The Tissue Distribution of Recombinant Adiponectin in Type 2 Diabetic Mice and Diet-induced Obesity Mice

To investigate the tissue distribution of 125I labeled adiponectin recombinant globular form(125I-gAcrp) in Diet-induced obesity(DIO) mice,type 2 diabetes mellitus(T2DM) mice and normal mice.125I-gAcrp was prepared and injected into different types of mice through the veins of tails,and then the radio-counts in brain,glandular angularis,heart,lung,liver,spleen,stomach,intestine,adipose tissue,kidney,muscle,testis,uterus and blood were measured at 0.5 and 2 h,respectively.The results showed that 125I-gAcrp has a much higher uptake at 2 h in the liver of T2DM mice and in the glandular angularis of DIO mice than that of at 0.5 h,whilst it has a lower uptake in the liver of DIO mice at 2 h,and also the uptake of 125I-gAcrp in the stomach,intestine and testis of DIO mice and in uterus of T2DM mice groups were increased.The result indicated that glandular angularis,testis and uterus probably play roles in the process of fat metabolism and blood glucose regulation of adiponectin in DIO and T2DM mice,besides liver and muscle.