A new cell line from human infiltrating ductal carcinoma of the breast: establishment and characterization |
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Authors: | W Zoli L Roncuzzi A Flamigni R Gruppioni A Sensi N Zini D Amadori A Gasperi-Campani |
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Institution: | (1) Department of Medical Oncology, G. B. Morgagni-L. Pierantoni Hospital, Viale Forlanini, 47100 Forli, Italy;(2) Biotechnology Unit, National Institute for the Research on Cancer (Genova), Bologna, Italy;(3) Department of Experimental Pathology, University of Bologna, Italy;(4) Istituto Oncologico Romagnolo, Forli, Italy;(5) Institute of Medical Genetics, Arcispedale S. Anna, University of Ferrara, Italy;(6) CNR Institute of Normal and Pathologic Cytomorphology, c/o I.O.R., Bologna, Italy;(7) Interdepartmental Center for Research on Cancer, University of Bologna, Italy |
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Abstract: | We established a novel cancer cell line (MAST) from the ascitic fluid of a metastatic infiltrating ductal carcinoma of the breast. The epithelial and neoplastic nature of the MAST cells was confirmed by ultrastructural analysis. The cell line was maintained as a monolayer with a doubling time of about 68 h, and it possessed an abnormal karyotype with a modal chromosome number of 60, a trisomy of chromosome 18 and other unidentified rearranged chromosomes. Among the markers consistently found in MAST metaphases, we noted a t(14; 14) and a very large subtelocentric, a large satellited acrocentric and a very large submetacentric chromosome with striking fluorescent bands. Immunoenzymatic assay demonstrated that the MAST cell line was positive for estrogen and progesterone receptors. The in vitro drug-sensitivity assay showed a marked resistance of the cell line to 5-fluorouracil and 4-hydroperoxycyclophosphamide and a moderate resistance to etoposide and 4 -epidoxorubicin. The molecular analysis showed a four- to sixfold amplification of the c-myc gene and no amplification or rearrangement of theint-2, c-erbB-2, c-Ha-ras, c-mos andhst-1 genes. |
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Keywords: | Human breast cancer Permanent cell line Cytogenetics oncogenes |
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