| 蚯蚓纤溶酶同工酶的分析 |
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| 引用本文： | 曹森,何执中,曾漪清,蒋企洲. 蚯蚓纤溶酶同工酶的分析[J]. 药物生物技术, 2003, 10(2): 100-101 |
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| 作者姓名： | 曹森 何执中 曾漪清 蒋企洲 |
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| 作者单位： | 1. 中国药科大学生物制药学院,南京,210009
2. 江苏省淡水水产研究所,南京,210017 |
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| 摘 要： | 对4种蚯蚓(赤子爱胜蚓、栉盲环毛蚓、通俗环毛蚓、威廉环毛蚓)进行粗提，将所得样品作活性印迹试验，观察同工酶谱以及经还原后谱带的变化情况，并对这4种蚯蚓活力大小进行比较，发现威廉环毛蚓总活力最高，其经过PAGE电泳后同工酶FE2活力最高。
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| 关 键 词： | 蚯蚓纤溶酶 同工酶 分析 PAGE电泳 |
| 文章编号： | 1005-8915(2003)02-0100-02 |
Analysis of EFE Isoenzyme |
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| CAO Sen ,HE Zhi-zhong ,ZENG Yi-qing ,JIANG Qi-zhou. Analysis of EFE Isoenzyme[J]. Pharmaceutical Biotechnology, 2003, 10(2): 100-101 |
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| Authors: | CAO Sen HE Zhi-zhong ZENG Yi-qing JIANG Qi-zhou |
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| Affiliation: | CAO Sen 1,HE Zhi-zhong 1,ZENG Yi-qing 2,JIANG Qi-zhou 1 |
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| Abstract: | Make active blotting experiments by what we get afte r extracting the four earthworms (Eisenia foelide, Pheretima pectinifera Michaelsea, Pheret ima vulgaris chen, Pheretima guillelmi)and a little share reduced by mercaptoethan ol ,and observe the difference of isoenzyme spectrums. Compared the activity of th e four earthworms, we concluded Pheretima guillelmi is the best for fibrino lyti c enzyme purification, then we further find the second isoenzyme in PAGE gel get s the highest activity . |
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| Keywords: | Earthworm fibrinolytic enzyme (EFE) Isoenzyme |
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