Ӥ�׶����Ը�к459����ԭ������

目的了解婴幼儿急性腹泻的病原构成,为本地区临床合理有效地控制小儿急性腹泻提供病原依据。方法对2009年1-12月福州儿童医院消化专科收治的459例急性腹泻患儿的粪便标本进行细菌培养,采用免疫胶体金法检测轮状病毒(RV)抗原;抽取其中218例,再应用ELISA法检测RV抗原,逆转录聚合酶链式反应(RT-PCR)法检测诺如病毒(NV)核酸。结果 459例患儿细菌感染35例(7.6%),真菌感染5例(1.1%),检出RV阳性117例(25.5%)。218例中RV阳性69例(31.7%),NV阳性61例(28.0%),上述二种病毒混合感染14例(6.4%)。两种病毒感染阳性率男女性别差异无统计学意义(P>0.05),不同年龄组间RV及NV阳性率差异有统计学意义(P<0.05),均以2岁以下患儿为主。RV、NV均有明显的季节特征,RV以10-12月份为发病高峰,NV以7~9月份为发病高峰分布,差异均有统计学意义(P<0.05)。结论小儿感染性腹泻病原类型复杂多样,在临床诊治中应重视病原学检测。

Pathogen isolation in 459 infants with acute diarrhea

Objective To study pathogen isolations from infants with acute diarrhea with the purpose to provide basic evidence for reasonable and effective control of infant acute diarrhea. Methods Totally 459 cases of acute diarrhea from Digestive Department in Fuzhou Children ’ s Hospital from Jan. to Dec. 2009 were selected , and their faeces samples were cultivated. Rotavirus ( RV ) was detected using Colloidal Gold method ; 218 cases were selected for RV detection again using ELISA method , norovirus nuclear acid were detected using RT-PCR method. Results Thirty-five of 459 cases were infected with bacteria , 5 cases were infected with fungi ( 1.1% ), and 117 cases were RV positive ( 25.5% ); 69 of 218 cases were RV postive ( 31.7% ), 61 cases were NV positive ( 28% ), and 14 cases were two viruses mixed infections ( 6.4% ) .The difference between male and female with two virus mixed infections had no statistical significance (P > 0.05 ); difference between different age groups in RV and NV positive rates had statistical significance (P < 0.05 ); all cases mainly were under 2 years old. RV and NV infections obviously had season difference , and RV infection peaked from October to December , while NV infection peaked from July to September , the difference having statistical significance (P < 0.05 ) . Conclusion The pathogenic types that cause infant infectious diarrhea are complex and diverse , thus we should pay attention to pathogen detection in clinical diagnosis and treatment.