胆囊结石研碎镜检法对华支睾吸虫感染检出率的研究

收集2010年1~6月于广州市番禺区第二人民医院普外科实施内镜微创取石保胆术的179例患者的血清、粪便、胆汁和胆囊结石标本,分别采用胶体金免疫层析法检测华支睾吸虫IgG抗体,粪便直接涂片法、胆汁离心沉渣镜检法和胆囊结石研碎镜检法检查华支睾吸虫虫卵,并进行比较分析。结果显示,胶体金免疫层析法检测华支睾吸虫IgG抗体的阳性率为51.4%(92/179),粪便直接涂片法、胆汁离心沉渣镜检法和胆囊结石研碎镜检法华支睾吸虫卵阳性检出率分别为30.7%(55/179)、44.7%(80/179)和69.8%(125/179),粪便直接涂片法检出率最低,胆囊结石研碎镜检法检出率最高(均P<0.05)。其中粪便直接涂片法和胆汁离心沉渣镜检法检测结果为阳性的患者,其结石研碎镜检法的检测结果也均为阳性。

Detection of Clonorchis sinensis Eggs in the Ground Gallbladder Stones by Microscopy

Sera,feces,bile and gallbladder stones were collected from 179 patients who accepted gallbladder-preserving cholelithotomy during the period of January to June 2010 at the general surgery department in the Second People′s Hospital of Panyu District in Guangzhou.Rapid colloidal gold immunochromatography was used to detect IgG against Clonorchis sinensis.C.sinensis eggs were examined by fecal direct smear,and in bile sediments and ground gallbladder stones.The results showed that the positive rate of rapid colloidal gold immunochromatographic assay for IgG was 51.4%,and the egg positive rate in feces,bile sediments and gallbladder stones was 30.7%,44.7% and 69.8%,respectively.The detection rate of fecal direct smear was the lowest,while that of the gallbladder stone examination was the highest(P<0.05).Those patients with egg-positive feces and/or bile sediments were all with egg-positive gallbladder stones.