S100B蛋白及NSE在婴儿痉挛症患儿脑脊液中的水平研究

目的探讨婴儿痉挛症(infantile spasm,IS)发病初期S100B蛋白、神经元特异性烯醇化酶(neuron specific enolase,NSE)在脑脊液中的浓度及与IS脑损伤的关系。方法采集23例婴儿痉挛症发病早期患儿脑脊液,应用ELISA方法定量检测S100B蛋白、NSE浓度;20名同年龄组非神经系统疾病患儿设为对照组。结果实验显示婴儿痉挛症患儿脑脊液中S100B蛋白水平高于对照组,差异有统计学意义(P<0.01);观察组神经元特异性烯醇化酶浓度显著高于对照组(P<0.01)。结论IS患儿发病初期脑脊液中S100B蛋白、NSE水平表达增高,提示痉挛发作可导致神经胶质细胞和神经元等多个部位的脑损伤,S100B蛋白和NSE可作为IS患儿脑损伤的生化标记。

Study on the levels of protein S100B and neuron-specific enolase in cerebrospinal fluid of infants with infantile spasm

Objective To investigate protein S100B and neuron-specific enolase levels in cerebrospinal fluid(CSF) during initial stages of infants with infantile spasm and assess the reletionship with cerebral injury.Methods CSF samples were collected from 23 infants with infantile spasm and from 20 control subjects(CON group).S100B and NSE concentrations were measured by enzyme-linked immunosorbent assay.Results Infants with infantile spasm had higher levels of S100B(P<0.01)and NSE(P<0.01)in CSF in comparison with those in control group.Conclusion Our finding of increased levels of S100B and NSE in infants with infantile spasm may be interpreted as evidence for cerebral injury.S100B and NSE can be biomarkers for cerebral injury in infants with infantile spasm.