中药复方861对二乙基亚硝胺诱发大鼠肝癌过程的影响

目的:观察中药复方861对二乙基亚硝胺(diethylnitrosamine,DEN)诱癌过程的影响.方法:250只雄性SD大鼠随机分成模型组、干预组、正常对照组.模型组和干预组以1?N灌胃,干预组同时以中药复方861灌胃.实验开始后分别于第2、3、5、8、10、12、14、18周分批处死大鼠,观察肝脏外观,肝组织进行HE、Masson三色染色,并对炎症坏死、肝纤维化程度和卵圆细胞增生程度进行评分,应用免疫组化及Western-blot方法检测肝组织中胎盘型谷胱甘肽硫转移酶(GST-P)蛋白的表达,计算成癌率.结果:干预组比模型组肝脏炎症坏死程度(P＜0.01)、纤维化程度(P＜0.01)和卵圆细胞增生程度(P＜0.05)轻;GST-P蛋白表达量少.结论:中药复方861可减轻DEN所致肝损伤.

Effect of Herbal Compound 861 on Hepatocarcinogenesis Induced by Diethylnitrosamine in Rats

Objective:To observe the effect of Herbal Compound 861 to the hepatocarcinogenesis induced by diethylnitrosamine in rats.Methods: 250 male SD rats were divided into normal group,model group and intervention group.The model and intervention group were administered 1% DEN intragastically once a week,for 14 weeks.The normal group was given saline instead of DEN,and intervention group were fed with Cpd 861 every day until the end of study.Seven to ten rats of model and intervention group were killed at 2,3,5,8,10,12,14,18 weeks,respectively.Four control rats were also killed at the same time points.The remained rats were followed to the end of the experiment at 26 weeks.Histopathological analysis were done by HE,Masson's trichrome of paraffin sections.The degree of necrosis,fibrosis and oval cell proliferation were analyzed.Immunohistochemistry and Western-blot was employed to detect the content of GST-P.Results: Cpd 861 intervention group had fewer nodules on outer surface of liver,less perivenular zone necrosis(P<0.01),fibrosis(P<0.01) and oval cell proliferation(P<0.05) than model group,and the level of GST-P protein were less in intervention group.Conclusion:Herbal compound 861 can reduce the severity of perivenular zone necrosis,fibrosis and oval cell proliferation induced by DEN,delay the emergence of liver cancer to some extent.