| 二仙汤提取物中3种成分含量测定的高通量方法学研究 |
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| 引用本文： | 朱霞,赵鑫,张巧艳,宋粉云,范国荣.二仙汤提取物中3种成分含量测定的高通量方法学研究[J].广东医药学院学报,2014(2):178-183. |
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| 作者姓名： | 朱霞 赵鑫 张巧艳 宋粉云 范国荣 |
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| 作者单位： | [1]安徽中医药大学药学院中药资源学教研室,安徽合肥230000 [2]第二军医大学药学院药物分析学教研室、上海市药物（中药）代谢产物研究重点实验室,上海200433 [3]第二军医大学药学院生药学教研室,上海200433 [4]广东药学院药科学院药物分析学教研室,广东广州510006 |
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| 基金项目： | 国家科技部重大新药创制专项（2010ZXJ0900X-003-003） |
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| 摘 要： | 目的 基于96孔板多功能酶标仪,建立二仙汤提取物中总黄酮、总皂苷及总生物碱含量测定的高通量分析方法.方法 分别以淫羊藿苷、菝葜皂苷元、盐酸小檗碱为对照品,采用96孔板比色法测定二仙汤提取物中总黄酮、总皂苷、总生物碱的质量分数.结果 淫羊藿苷、菝葜皂苷元、盐酸小檗碱分别在41.12～205.6、81.12～405.6、20.34～ 61.02 μg/mL内线性关系良好,加样回收率分别为98.3％（RSD为4.44％,n=6）、101.3％（RSD为1.55％,n=6）、100.9％（RSD为2.15％,n=6）.结论 96孔板比色法简单快速、通量高、准确度高、重复性好,可以用于大样本量二仙汤提取物生产过程的质量控制.
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| 关 键 词： | 二仙汤提取物 总黄酮 总皂苷 总生物碱 96孔板 比色法 |
Study on high-throughput methodology of quantitative determination of three compositions in Er-xian decoction extract |
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| Authors: | ZHU Xia ZHAO Xin ZHANG Qiaoyan SONG Fenyun FAN Guorong |
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| Institution: | 1.Department of Traditional Chinese Resources, School of Pharmacy ,Anhui University of Chinese Medicine, Hefei 230000, China; 2. Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University/Shanghai Key Laboratory for Pharmaceutical (Chinese Materia Medica )Metabolite Research, Shanghai 200433, China ; 3. Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China; 4. Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China ) |
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| Abstract: | Objective To establish a high-throughput method of quantitative determination for total flavonoids,total saponins and total alkaloids in Er-xian decoction based on 96-well plates Varioskan Flash multimode microplate reader. Methods 96-well plates colorimetry was used for the determination of total flavonoids, total saponins and total alkaloids in Er-xian decoction extract, with icariin, sarsasapogenine and berberine hydrochloride as reference substances, respectively. Results The colorimetry showed good specificity for quantitative analysis of total flavonoids, total saponins and total alkaloids in the extract of Er- xian decoction.The linear range of icariin, sarsasapogenin and berberine hydrochloride was 41.12-205.6 μg/ mL,81.12-405.6 μg/mL and 20.34-61.02 μg/mL, respectively.The average recoveries were 98.3% （RSD = 4. 44%, n = 6） for icariin, 101.3% （ RSD = 1.55%, n = 6） for sarsasapogenin and 100. 9% （ RSD = 2. 15% ,n= 6） for berberine hydrochloride.Conelusion 96-well plates eolorimetry was simple and rapid, high-throughput,highly accurate, reproducible, and suitable for large sample analysis in quality control of Er-xian decoction. |
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| Keywords: | Er-xian decoction extract total flavonoids total saponins total alkaloids 96-well plates colorimetry |
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