| C57胚胎小鼠神经干细胞的分离、培养与鉴定 |
| |
| 引用本文： | 万丽,易桦林,贝伟剑. C57胚胎小鼠神经干细胞的分离、培养与鉴定[J]. 广东医药学院学报, 2014, 0(3): 354-357 |
| |
| 作者姓名： | 万丽 易桦林 贝伟剑 |
| |
| 作者单位： | [1]广东药学院中医药研究院／广东省代谢性疾病中医药防治重点实验室,国家中医药管理局高脂血症调肝降脂重点研究室／国家中医药管理局脂代谢三级实验室,广东广州510006 [2]中山大学生命科学学院干细胞研究室,广东广州510006 |
| |
| 基金项目： | 广东省教育部产学研结合项目（2012B091100193）; 广州市科技计划助资项目（11C32130738） |
| |
| 摘 要： | 目的体外分离、培养神经干细胞（NSCs）,并对其进行鉴定。方法采用手动法及胰蛋白酶消化法分离胎鼠脑细胞,用优化的无血清NSCs培养基进行培养。细胞免疫荧光法检测NSCs特异性标记分子的表达。结果分离的脑细胞体外培养48 h已大部分贴壁,3 d左右获得大量未分化呈巢状悬浮生长的神经干细胞团。第3代时,很少见到贴壁细胞,几乎全是神经球,神经球周围存在较多微刺。细胞表达一种中间丝蛋白,即巢蛋白（nestin）。结论成功分离、鉴定出C57小鼠NSCs,并可在体外条件下进行传代扩增培养。
|
| 关 键 词： | C57胚胎小鼠 神经干细胞 细胞培养 巢蛋白 |
Isolation,culture and identification of neural stem cells from C57 embryonic mice |
| |
| WAN Li,YI Hualin,BEI Weijian. Isolation,culture and identification of neural stem cells from C57 embryonic mice[J]. , 2014, 0(3): 354-357 |
| |
| Authors: | WAN Li YI Hualin BEI Weijian |
| |
| Affiliation: | 1. Guangdong TCM Key Laboratory for Metabolic Diseases, Key Laboratory of Modulating Liver to Treat Hyperlipemia SATCM, and Level 3 Laboratory of Lipid Metabolism SATCM, Institute of Chinese Medicinal Sciences, Guangdong Pharmaceutical University, Guangzhou 510006, China; 2. Stem Cell Research Department, School of Life Sciences, Sun Yatsen University, Guangzhou , 510006, China) |
| |
| Abstract: | Objective To isolate,culture and identify the neural stem cells（NSCs）.Methods NSCs from fetal mice were isolated by dissection and enzymatic digestion,and cultured in the optimal serum-free NSC medium.The specific biomarker of NSCs was detected by immunocytofluorescent assay.Results The isolated NSCs adhered to the culture plate within 48 h,and nest-like clusters of NSCs were obtained in the culture suspension in about 3 d.On the 3th passages of NSCs,adherent cells are rarely seen,and nearly all were neurospheres with spinule.Nestin was expressed in the cultured cells.Conclusion NSCs are successfully isolated and cultured from fetal C57 mice,and could proliferate in vitro. |
| |
| Keywords: | C57 embryonic mice neural stem cells cell culture nestin |
| 本文献已被 维普 等数据库收录！ |
|
